Expression of KCC4 in distal colon surface epithelia

KCC4在远端结肠表面上皮细胞中的表达

• 批准号: 7057461
• 负责人: Colleen Rebecca Talbot
• 金额: $ 4.83万
• 依托单位: UNIVERSITY OF CALIFORNIA RIVERSIDE
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-01-01 至 2007-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7057461
• 关键词: colon; dietary potassium; dietary sodium; enzyme activity; gastrointestinal absorption /transport; gene expression; hydrogen potassium exchanging ATPase; immunologic substance development /preparation; laboratory mouse; laboratory rabbit; laboratory rat; membrane permeability; membrane transport proteins; nutrition related tag; polymerase chain reaction; potassium channel; potassium ion; protein localization; western blottings

DESCRIPTION (provided by applicant): The long-term objective of this proposal is to understand the role of the K+CI- co-transporter, KCC4, in hormonal and dietary control of distal colon K+ reabsorption and how it is used regulating whole body K+ homeostasis. An antibody against rat KCC4 will be characterized and used to study cellular expression of this transporter along the proximal to distal axis of rat colon. The specific aims of this proposal include: 1) fully characterize and optimize an anti-KCC4 antibody to maximize its effectiveness in detecting and localizing KCC4 expression in rat; 2) perform real time PCR, Western blotting and immunolocalization studies to examine expression of KCC4 mRNA and protein in rat distal colon epithelia; and 3) compare effects of dietary restriction of K+ and Na+ on expression of KCC4, KCC1 and colonic H/K-ATPase. These techniques will be brought back to the Fellows home campus, an hispanic-serving institution, where they will be used to train (minority) students who are interested in careers in the health professions and/or biomedical research. It is anticipated that a long-term collaboration will be established between the Fellow and the Sponsor, further helping to track these students to research careers at a major doctoral granting institution.

描述（由申请人提供）：本提案的长期目标是了解K+CI-协同转运蛋白KCC 4在远端结肠K+重吸收的激素和饮食控制中的作用，以及如何使用其调节全身K+稳态。抗大鼠KCC 4的抗体将被表征并用于研究该转运蛋白沿着大鼠结肠近端至远端轴的细胞表达。本研究的具体目标包括：1）充分表征和优化抗KCC 4抗体，以最大化其在检测和定位大鼠中KCC 4表达中的有效性; 2）进行真实的时间PCR、蛋白质印迹和免疫定位研究，以检测大鼠远端结肠上皮中KCC 4 mRNA和蛋白的表达;比较限钾、限钠对KCC 4、KCC 1和结肠H/K-ATP酶表达的影响。这些技术将被带回研究员的家乡校园，一个为西班牙裔服务的机构，在那里他们将被用来培训（少数民族）学生谁有兴趣在卫生专业和/或生物医学研究的职业生涯。预计研究员和赞助商之间将建立长期合作，进一步帮助跟踪这些学生在一个主要的博士学位授予机构的研究生涯。