USING VIRAL NANOPARTICLES TO TARGET CANCER

使用病毒纳米颗粒靶向癌症

• 批准号: 7003678
• 负责人: MARIANNE MANCHESTER
• 金额: $ 72.69万
• 依托单位: SCRIPPS RESEARCH INSTITUTE, THE
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-03-01 至 2010-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7003678
• 关键词: CHO cells; Comovirus; athymic mouse; bioengineering /biomedical engineering; early diagnosis; enzyme linked immunosorbent assay; imaging /visualization /scanning; introns; molecular biology; nanotechnology; neoplasm /cancer diagnosis; protooncogene; tissue /cell culture; virus assembly; virus genetics; viruslike particle; western blottings

DESCRIPTION (provided by applicant): Nanotechnology holds great promise for the early detection and treatment of cancer. The ability to both detect and follow the initiation and progression of cancer without biopsy, surgery, or other invasive techniques should lead to lower costs and higher quality of life. The goal of this proposal is to develop viral nanoparticles (VNPs) as platforms for combination tumor targeting and imaging agents in vivo. Our multidisciplinary team combines molecular biology, structure, chemistry and in vivo biology to attack this problem. In this proposal we will study two well-characterized viruses: a plant virus, cowpea mosaic virus (CPMV); and an insect virus, flockhouse virus (FHV). The accumulated knowledge of the structure, function, assembly, genome packaging, chemical attachment, and in vivo bioavailability of FHV and CPMV, developed in the co-investigators' laboratories, makes these viruses ideal candidates for such platform development. We will test the ability of tumor-specific VNPs to attack tumor cells in vitro and in vivo, and study mechanisms of uptake of VNPs into cells. We will also package anti-tumor compounds and inhibitory genes inside the particles to facilitate tumor destruction. Finally we will test the ability of our VNPs to detect and destroy tumors in vivo. These studies will make a significant contribution to the development of improved tumor targeting and imaging nanotechnologies.

描述（由申请人提供）：纳米技术对癌症的早期发现和治疗有很大的希望。在没有活检，手术或其他侵入性技术的情况下检测和遵循癌症的启动和进展的能力应导致降低成本和更高的生活质量。该建议的目的是开发病毒纳米颗粒（VNP）作为体内肿瘤靶向和成像剂的平台。我们的多学科团队结合了分子生物学，结构，化学和体内生物学，以攻击这个问题。在此提案中，我们将研究两种特征性的病毒：一种植物病毒，牛皮镶嵌病毒（CPMV）；还有一种昆虫病毒，羊绒病毒（FHV）。在共同投资者的实验室中开发的FHV和CPMV的结构，功能，组装，基因组包装，化学依恋以及体内生物利用度的积累知识使这些病毒成为这种平台开发的理想候选者。我们将测试肿瘤特异性VNP在体外和体内攻击肿瘤细胞的能力，以及研究VNP摄取VNP的机制。我们还将包装颗粒内部的抗肿瘤化合物和抑制基因，以促进肿瘤破坏。最后，我们将测试VNP检测和破坏体内肿瘤的能力。这些研究将为改善肿瘤靶向和成像纳米技术的发展做出重大贡献。