Pox Proteomics

描述（由申请人提供）：本探索性研究项目的总体目标是对牛痘病毒（VV）病毒粒子进行蛋白质组学分析，为制定新的抗病毒干预策略和预防方案提供基础，以预防天花等正痘病毒引起的疾病。自1990年以来，VV基因组已被测序(1)，但大约一半基因的作用仍然完全未知。虽然对牛痘病毒的许多蛋白质进行了详细的研究，但目前还没有对牛痘病毒颗粒进行全面的蛋白质组学分析。为了进一步了解VV病毒和其他致病性更高的正痘病毒家族成员的复制周期，并发现可能成为新抗病毒药物靶点或新一代疫苗抗原的蛋白质，我们为本研究项目确定了两个具体目标：采用质谱法建立细胞内成熟病毒（IMV）病毒特异性蛋白质组参考目录；2)。对病毒粒子蛋白上的翻译后修饰（PTMs）进行蛋白质组学分析，首先关注磷酸化蛋白和酰基蛋白。人们期望通过对病毒蛋白组的分析，获得新的病毒基因产物的表达和定位信息。未来的工作可以集中在它们的隔离和深入表征上。VV病毒粒子形式的综合蛋白质组参考图的可用性对病毒病毒学家来说将是无价的，并将为病毒性疾病的新预防和治疗策略提供关键数据。

DESCRIPTION (provided by applicant): The overall goal of this exploratory research project is to conduct a proteomic analysis of the vaccinia virus (VV) virion, which will provide the foundation for the development of new antiviral intervention strategy and prevention programs to prevent diseases caused by orthopoxviruses such as smallpox. The VV genome has been sequenced since 1990 (1), but the roles of about half of the genes have remained entirely unknown. Although, numerous proteins of vaccinia virus have been studied in great detail, there no comprehensive proteomic analysis of the VV particle is available. To advance our understanding of the replication cycle of the VV and other more pathogenic members of the orthopoxvirus family and to discover proteins that may become targets for a new antiviral drugs or antigens for a new generation of vaccines we define two specific aims for this research project: 1.) Establish virion-specific proteome reference catalogs for the intracellular mature virus (IMV) by using mass spectrometry; 2.) Perform proteomic analysis of post-translational modifications (PTMs) present on virion proteins, focusing initially on phosphoproteins and acylproteins. It is expected that the proteome analysis of VV will yield information on the expression and location of new viral gene products. Future efforts can then focus on their isolation and in-depth characterization. The availability of comprehensive proteome reference maps of the VV virion forms will be invaluable for poxvirologists and will provide key data for novel prevention and treatment strategies for viral diseases.