Array-microscope Multispectral Whole Slide Imager

描述(申请人提供)：项目摘要/摘要：我们建议为全幻灯片多光谱幻灯片扫描仪开发一种创新的多对一光学系统，以快速生成病理上的虚拟幻灯片。该光学系统的主要用途是实现组织和组织微阵列载玻片的多光谱成像。多光谱成像是推进基于组织病理分析的诊断和预后的关键进展。多光谱成像起源于遥感。在显微镜的背景下，这仍然是一个新的发展。在遥感和显微镜方面，多光谱成像的主要优势是分别对光谱重叠的矿物或分子特征进行数字分解。这里提出的光学系统将允许以数值孔径NA=0.65的单次扫描扫描显微镜载玻片，以0.47微米的采样覆盖整个载玻片的宽度和长度。此外，拟议的多光谱幻灯片扫描仪将在整个幻灯片图像中的每个(0.47微米)2像素处提供至少16个连续的光谱波段测量。所有多光谱数据将在10秒内收集到S，比现有的全载玻片扫描仪快5倍或更多，现有的全载玻片扫描仪只能捕获每个样本3种颜色。目前，市场上还没有类似的产品。第一阶段项目侧重于仪器原型的设计和组装。对样机的评估强调图像质量、光学传输效率和收集的多光谱数据的准确性。原型的测量结果将与我们的两位合作者获得的多光谱成像结果进行比较，以确定其一致性。在第二阶段，我们计划开发一种模块化的“工业”阵列显微镜仪器，用于快速多光谱成像，适用于Brightfield和表观荧光成像模式。项目简介：多光谱技术与Dmetrix的超快速、自动化成像技术相结合，代表着推进基于组织的诊断和预后病理分析的关键发展。外科病理学家将使用多光谱成像技术来确定肿瘤病因学、多参数分析和预后以及治疗药物决定。

DESCRIPTION (provided by applicant): Project Summary/Abstract: We propose to develop an innovative "many-to-one" optical system for a whole-slide multispectral slide scanner to rapidly generate virtual slides in pathology. A primary use of this optical system is to enable multispectral imaging of tissue and tissue-microarray slides. Multispectral imaging is a key development in advancing diagnostic and prognostic tissue-based pathology analysis. Multispectral imaging has its roots in remote sensing. In the context of microscopy, it is still a recent development. The main advantage of multispectral imaging, in remote sensing as well as in microscopy, is the digital unmixing of spectrally overlapping mineral or molecular signatures, respectively. The optical system proposed here will allow scanning a microscope slide in a single sweep at a numerical aperture of NA = 0.65, covering the entire width and length of a slide with 0.47-um sampling. In addition, the proposed multispectral slide scanner will deliver at least 16 contiguous spectral-band measurements at each (0.47 um)2 pixel in the whole-slide image. All multispectral data will be collected in 10's of seconds, 5 or more times faster than existing whole-slide scanners which only capture 3 colors per specimen. At this time, there is no equivalent product on the market. The Phase I project emphasizes design and assembly of an instrument prototype. The evaluation of the prototype emphasizes image quality, optical transfer efficiency, and accuracy of collected multispectral data. Measurements taken with the prototype will be compared for consistency with multispectral-imaging results obtained by our 2 collaborators. In Phase II, we plan to develop a modular, "industrial" array-microscope instrument for rapid multispectral imaging, applicable in brightfield and epi-fluorescence imaging modalities. Project Narrative: Multispectral technology, combined with Dmetrix's ultra-rapid, automated imaging technology, represents a key development in advancing diagnostic and prognostic tissue-based pathology analysis. Multispectral imaging technology will be used by the surgical pathologist to define tumor etiology, multiparameter assays and prognostics as well as therapeutic drug decisions.