Focusing Differential Mobility Analyzer

聚焦差分迁移率分析仪

• 批准号: 7106838
• 负责人: Victor Laiko
• 金额: $ 9.91万
• 依托单位: SCIENCE AND ENGINEERING SERVICES, INC.
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-06-01 至 2007-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7106838
• 关键词: ions

DESCRIPTION (provided by applicant): Ion Mobility Spectrometer coupled with electrospray ion source and interfaced to a Mass Spectrometer is a powerful tool for the study of molecular conformations, separation of mass isomers, and analysis of complex mixtures and suppression of chemical background. One of the reasons that limit the capabilities of the technique is its relatively low resolving power. The gas-phase diffusion results in the peak broadening and limits the resolution at 50-100 level. We propose the alternative principle of gas-phase ion separation, based on ion focusing under the influence of uniform electrostatic field and decelerating gas flow. Both analytical calculations and numerical simulation show that a diffusion-limited resolution of several hundred can be achieved. We propose to build an ion mobility analyzer of the new type that is called Focusing Differential Mobility Analyzer (FDMA). The proposed prototype FDMA can be easily interfaced with commercial mass spectrometers and offers the theoretical resolution of several hundred and ion transmission close to 100%. The purpose of this proposal is the demonstration of the feasibility of atmospheric-pressure ion separation based on the new principle of ion focusing under the influence of uniform electrical field and decelerating gas flow. A prototype FDMA apparatus will be designed and built. The electrospray ion source will be coupled with FDMA. The devices will be interfaced with commercial LCQ ion trap of Thermo Finnigan, Inc., installed in our laboratory. The success of the research will provide a scientific community with a new class of ion mobility analyzer that combines better resolution and sensitivity with simplicity of design and interface to mass spectrometers of different types.

描述（由申请人提供）：与电喷雾离子源耦合并与质谱仪连接的离子迁移谱仪是研究分子构象、分离质量异构体、分析复杂混合物和抑制化学背景的有力工具。限制该技术能力的原因之一是其相对较低的分辨率。气相扩散导致峰宽变宽，使分离度限制在50-100级。我们提出了气相离子分离的替代原则，离子聚焦的影响下，均匀的静电场和减速气流。分析计算和数值模拟表明，扩散限制的分辨率可以达到几百。我们建议建立一个新型的离子迁移率分析仪，称为聚焦差分迁移率分析仪（FDMA）。所提出的原型FDMA可以很容易地与商业质谱仪接口，并提供了数百和离子传输接近100%的理论分辨率。本提案的目的是论证基于均匀电场和减速气流影响下的离子聚焦新原理的大气压离子分离的可行性。设计并制造了一个原型频分多址装置。电喷雾离子源将与FDMA耦合。这些装置将与Thermo Finnigan，Inc.的商用LCQ离子阱连接，安装在我们的实验室里。这项研究的成功将为科学界提供一种新型的离子迁移率分析仪，该分析仪将更好的分辨率和灵敏度与简单的设计和不同类型质谱仪的接口相结合。