Spatial Mapping Of Gene Expression Early Mouse Embryo

这个项目的目的是绘制特定基因在植入前小鼠胚胎中表达的位置。Ko博士在LG的团队使用微阵列的表达谱来识别在小鼠早期胚胎发育的不同阶段差异表达的500-1000个基因。 吉川博士成功地开发了用于原位杂交和显微镜下处理整装胚胎的中等产量技术。他能够应用这些技术来定位91个候选基因的位置，这些基因是在胚泡中高表达的转录因子。在91例中，48例主要表达于内细胞团。这些基因主要在ICM中的表达表明它们在维持多能性方面的重要性。 该项目现在分为发育基因组学和老年科(DGAS)和图像信息学和计算生物学股(IICBU)两个独立的项目。在IICBU内，这一项目(开放显微镜环境的开发和应用)的继续涉及为可公开访问的原位杂交图像储存库建立信息学基础设施，以及开发注释和分析这些图像的工具。在DGA中，该项目的继续包括分析选定的发育基因在小鼠早期胚胎和胚胎干细胞中的表达模式的空间分布。

The purpose of this project is to map the locations at which specific genes are expressed in pre-implantation mouse embryos. Dr. Minoru Ko's group in LG has used expression profiling with microarrays to identify 500-1000 genes that are differentially expressed at various stages of early mouse embryo development. Dr. Yoshikawa was successful at developing medium-throughput techniques for processing whole-mount embryos for in situ hybridization and microscopy. He was able to apply these techniques to map the locations of 91 candidate genes that were transcription factors highly expressed in blastocysts. Of these 91, 48 were found to be expressed predominantly in the inner-cell mass. The expression of these genes predominantly in the ICM suggests their importance in maintaining pluripotency. This project is now split into separate projects at the Developmental Genomics and Aging Section (DGAS) and the Image informatics and Computational Biology Unit (IICBU). Within the IICBU, the continuation of this project (Development And Applications Of The Open Microscopy Environment) involves establishing the informatics infrastructure for a publicly accessible repository of in-situ hybridization images as well as the development of tools to annotate and analyze these images. Within DGAS, continuation of this project involves assaying spatial distribution of expression patterns of selected developmental genes in the early mouse embryo and embryonic stem cells.