Spatial Mapping Of Gene Expression Early Mouse Embryo

早期小鼠胚胎基因表达的空间图谱

• 批准号: 7132311
• 负责人: David Schlessinger
• 金额: --
• 依托单位: NATIONAL INSTITUTE ON AGING
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7132311
• 关键词: animal genetic material tag; developmental genetics; embryogenic cleavage; fluorescent in situ hybridization; gene expression; genetic screening; laboratory mouse; mammalian embryology; microarray technology; microscopy; pluripotent stem cells; transcription factor

The purpose of this project is to map the locations at which specific genes are expressed in pre-implantation mouse embryos. Dr. Minoru Ko's group in LG has used expression profiling with microarrays to identify 500-1000 genes that are differentially expressed at various stages of early mouse embryo development. Dr. Yoshikawa was successful at developing medium-throughput techniques for processing whole-mount embryos for in situ hybridization and microscopy. He was able to apply these techniques to map the locations of 91 candidate genes that were transcription factors highly expressed in blastocysts. Of these 91, 48 were found to be expressed predominantly in the inner-cell mass. The expression of these genes predominantly in the ICM suggests their importance in maintaining pluripotency. This project is now split into separate projects at the Developmental Genomics and Aging Section (DGAS) and the Image informatics and Computational Biology Unit (IICBU). Within the IICBU, the continuation of this project (Development And Applications Of The Open Microscopy Environment) involves establishing the informatics infrastructure for a publicly accessible repository of in-situ hybridization images as well as the development of tools to annotate and analyze these images. Within DGAS, continuation of this project involves assaying spatial distribution of expression patterns of selected developmental genes in the early mouse embryo and embryonic stem cells.

该项目的目的是绘制特定基因在植入前小鼠胚胎中表达的位置。LG的Minoru Ko博士的研究小组使用微阵列的表达谱来识别在早期小鼠胚胎发育的各个阶段差异表达的500-1000个基因。 博士Yoshikawa成功地开发了中等通量的技术，用于处理原位杂交和显微镜检查的整装胚胎。他能够应用这些技术来绘制91个候选基因的位置，这些基因是在胚泡中高度表达的转录因子。在这91个中，发现48个主要在细胞内团中表达。这些基因主要在ICM中表达，表明它们在维持多能性中的重要性。 这个项目现在被分成发育基因组学和衰老科（DGAS）和图像信息学和计算生物学单位（IICBU）的单独项目。在IICBU内，该项目的继续（开放显微镜环境的开发和应用）涉及建立信息学基础设施，用于公开访问原位杂交图像库，以及开发注释和分析这些图像的工具。在DGAS内，该项目的继续涉及分析早期小鼠胚胎和胚胎干细胞中选定发育基因表达模式的空间分布。