VISION RESEARCH INFRASTRUCTURE DEVELOPMENT GRANT

愿景研究基础设施发展补助金

• 批准号: 7068464
• 负责人: ANTHONY BART NESBURN
• 金额: $ 22.88万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-06-01 至 2010-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7068464
• 关键词: microscopy; tissue /cell culture; vision

DESCRIPTION (provided by applicant): A Vision Research Infrastructure Development Grant to support vision research within the Department of Ophthalmology at the University of California, Irvine is proposed to provide funding of shared equipment and resources and to promote greater collaboration between vision scientists. Recent growth within the Department has brought together cellular and molecular biologists, immunologists, virologists and engineers that share an interest in studying vision related disease mechanisms. The goals of this proposal are two fold: (1) To bring commonly used technologies together within a shared environment to provide a more efficient mechanism for supporting existing equipment and expertise. (2) To bring together individual investigators from diverse disciplines to provide an environment for collaborative multidisciplinary research that maximally utilizes the individual capabilities of the investigators within the Department of Ophthalmology. In order to achieve these goals we have identified two key functions, (1) Cell Culture; and 2) Microscopic Imaging, that not only need current support but will also greatly expand our abilities to answer vision related questions in the future. We therefore propose to establish two Core Facilities. First, the Cell Culture Facility will be established to a) maintain shared tissue culture equipment and services, b) provide routine cell culture services, c) cell banking, d) mycoplasma and endotoxin monitoring, and e) Vi-CELL(tm) Viability Analyzing. Secondly, the Microscopy Imaging Facility that will provide state-of-the-art services in a) light and fluorescent microscopy, b) confocal laser scanning microscopy, c) multiphoton confocal microscopy and time lapse imaging, d) in vivo confocal microscopy and e) quantitative morphometry and digital image analysis. While the immediate goal of this Infrastructure Development Grant is to establish more centralized facilities to save time, energy and resources, we believe that establishing these facilities will greatly expand the individual capabilities of each investigator through enhanced collaboration with other investigators from different disciplines and by providing easier access to state-of-the-art equipment and the expertise needed to fully use these new technologies.

描述(由申请者提供)：为支持加州大学欧文分校眼科系内的视觉研究，建议设立视觉研究基础设施发展基金，为共享设备和资源提供资金，并促进视觉科学家之间的更大合作。该部门最近的发展使细胞和分子生物学家、免疫学家、病毒学家和工程师聚集在一起，他们对研究与视觉有关的疾病机制有共同的兴趣。这项提议的目标有两个：(1)将常用技术集中在一个共享环境中，为支持现有设备和专门知识提供更有效的机制。(2)汇聚来自不同学科的个别研究人员，为多学科合作研究提供一个环境，以最大限度地利用眼科内调查人员的个别能力。为了实现这些目标，我们已经确定了两项关键功能，(1)细胞培养；2)显微成像，这不仅需要目前的支持，而且将极大地扩展我们未来回答与视觉有关的问题的能力。因此，我们建议设立两个核心设施。首先，将建立细胞培养设施，以a)维护共享的组织培养设备和服务，b)提供常规的细胞培养服务，c)细胞库，d)支原体和内毒素监测，以及e)Vi-cell(TM)活性分析。第二，显微成像设备将在a)光学和荧光显微镜、b)共焦激光扫描显微镜、c)多光子共焦显微镜和时间推移成像、d)活体共焦显微镜以及e)定量形态测量和数字图像分析方面提供最先进的服务。虽然这笔基础设施发展赠款的直接目标是建立更多的集中化设施，以节省时间、能源和资源，但我们相信，建立这些设施将通过加强与来自不同学科的其他研究人员的合作，并通过提供更容易获得最先进的设备和充分使用这些新技术所需的专业知识，来极大地扩大每个调查员的个人能力。