CONSTRUCTION OF A TARGETED RHESUS MACAQUE MICROARRAY

靶向恒河猴微阵列的构建

• 批准号: 7276957
• 负责人: ROBERT B. NORGREN
• 金额: $ 8.34万
• 依托单位: UNIVERSITY OF NEBRASKA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-06-18 至 2008-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7276957
• 关键词: CONSTRUCTION; TARGETED; RHESUS; MACAQUE; MICROARRAY

DESCRIPTION (provided by applicant): The rhesus macaque (Macaca mulatta) offers unique opportunities for biomedical research due to similarities to humans in both anatomy and physiology. Vaccine development for HIV and possible bioterrorist agents such as small pox and anthrax require the use of nonhuman primates (NHPs). The rhesus macaque is also the animal of choice for studies of reproductive biology and higher cortical functions. Important studies in these areas are currently impeded by the lack of a critical tool - expression microarrays. The objective of this proposal is to create rhesus macaque expression microarrays by targeted polymerase chain reaction (PCR) amplification of monkey homologs of human genes using information from the human genome project. To maximize scientific and biomedical impact, this targeted approach will be prioritized in three ordered stages. The first stage will be to produce a focused array of 1,800 genes chosen as highest priority by the NHP community. The second stage will be to identify and amplify those monkey genes for which human probes work poorly. The third stage will be to amplify the remaining rhesus macaque genes. Genes already cloned from rhesus cDNA libraries will be excluded from this project. Each stage will have four steps: (1) identify the genes; (2) design primer pairs for targeted amplification of those genes; (3) amplify, subclone, sequence, annotate, and deposit those gene clones; and (4) construct targeted rhesus expression microarrays. This project will produce a resource of major importance to the NHP community.

描述（由申请人提供）： 恒河猴（Macaca mulatta）由于在解剖学和生理学上与人类相似，为生物医学研究提供了独特的机会。 艾滋病毒和可能的生物恐怖分子制剂（如天花和炭疽）的疫苗开发需要使用非人灵长类动物（NHP）。 恒河猴也是研究生殖生物学和高级皮质功能的首选动物。 这些领域的重要研究目前由于缺乏关键工具-表达微阵列而受到阻碍。 本提案的目的是利用人类基因组计划提供的信息，通过靶向聚合酶链反应（PCR）扩增人类基因的猴同源基因，创建恒河猴表达微阵列。 为了最大限度地扩大科学和生物医学影响，将分三个有序阶段优先考虑这一有针对性的方法。 第一阶段将产生由NHP社区选择的最高优先级的1，800个基因的集中阵列。 第二阶段将是识别和扩增那些人类探针效果不佳的猴子基因。 第三阶段将是扩增剩余的恒河猴基因。 已经从恒河猴cDNA文库中克隆的基因将被排除在本项目之外。 每个阶段将有四个步骤：（1）鉴定基因;（2）设计用于靶向扩增这些基因的引物对;（3）扩增、亚克隆、测序、注释和存款这些基因克隆;和（4）构建靶向恒河猴表达微阵列。 该项目将产生对NHP社区具有重大意义的资源。