Laser Scanning Confocal Microscope

描述（由申请人提供）：资金要求购买激光扫描共聚焦显微镜。该系统是Zeiss LSM 510 Meta与Axiovert 200 MOT相结合，将取代已有10年历史的LSM 410系统，该系统有29个部门的232个注册用户。所要求的系统将被安置和神经科学成像中心（NIC），在我们大学最古老的成像核心设施管理。NIC聘请了一名拥有8年操作LSM 410共聚焦显微镜经验的全职技术人员。描述了8个主要用户和9个次要用户的项目。这些项目将受益于LSM 510 Meta改进的光学和检测系统，从而改善对荧光染料发射的捕获、光谱分离、降低光毒性以及进行荧光共振能量转移（FRET）测量的能力。主要用户的项目包括：大鼠和小鼠海马脑片及培养物中Ca ~（2+）通道的运输（Dr. Herlitze），肌球蛋白II在人类癌细胞运动过程中的作用和机械功能（Egelhoff博士），开发新的小鼠品系，作为脑多巴胺能神经元研究的遗传工具（Dr. Deneris），调节5-HT受体靶向和贩运（博士罗斯），作用的动力相互作用蛋白质的内吞周期在果蝇黑腹（罗宾逊博士）、内吞作用在EGF受体运输中的作用（卡林博士）、出生后小脑分化的调节（西格尔博士）和神经胶质瘢痕轴突再生（银博士）。

DESCRIPTION (provided by applicant): Funds are requested to purchase a laser scanning confocal microscope. This system, the Zeiss LSM 510 Meta coupled to an Axiovert 200 MOT, will replace a 10-year-old LSM 410 system with 232 registered users from 29 departments. The requested system will be housed and administered by the Neuroscience Imaging Center (NIC), the oldest imaging core facility at our University. The NIC employs a full-time technician with 8 years of experience operating the LSM 410 confocal microscope. Projects from 8 major and 9 minor users are described. These projects will benefit from the improved optics and detection system of the LSM 510 Meta, resulting in improved capture of fluorochrome emission, spectral separation, reduced phototoxicity and the ability to do fluorescence resonance energy transfer (FRET) measurements. Projects of the major users include: Ca 2+ channel trafficking in hippocampal slices or cultures of rat and mice (Dr. Herlitze), role and mechanical function of myosin II during cell locomotion in human cancer cells (Dr. Egelhoff), development of new mouse strains as genetic based tools for brain serotonergic neuron studies (Dr. Deneris), regulation of 5-HT-receptor targeting and trafficking (Dr. Roth), role of dynamin interacting proteins in the endocytotic cycle in Drosophila melanogaster (Dr. Robinson), role of endocytosis in EGF receptor trafficking (Dr. Carlin), regulation of postnatal cerebellar differentiation (Dr. Siegel) and axon regeneration across glial scar (Dr. Silver).