Nano-HPLC-ESI Quadrupole Ion Trap Mass Spectrometer

Nano-HPLC-ESI 四极杆离子阱质谱仪

• 批准号: 6876889
• 负责人: Alan V. Smrcka
• 金额: $ 34.04万
• 依托单位: UNIVERSITY OF ROCHESTER
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-04-15 至 2006-10-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6876889
• 关键词: biological signal transduction; biomedical equipment purchase; electrospray ionization mass spectrometry; microarray technology; protein sequence; proteomics

DESCRIPTION (provided by applicant): We request funds for the purchase of an electrospray-ionization ion trap mass spectrometer for protein and proteome analysis. The specific instrument is a Thermo Finnigan LTQ linear quadrupole ion trap mass spectrometry system coupled to a Surveyor HPLC system capable of 100 nL to uL flow rates and a nano or electrospray source. No instrument with comparable capabilities is available at the University of Rochester. There is a need for this instrument as demonstrated by eight major users and at least two minor users, all of whom are funded by NIH. Alan V. Smrcka (Principal Investigator) has been using hydrogen/deuterium exchange MALDI-TOF mass spectrometry for analysis of signal transduction proteins. Acquiring this instrumentation would greatly advance the level of this analysis. Other major users propose to use the system to study global protein modifications in yeast (E. Phizicky), proteins involved in nuclear transport of histones (J. Hayes), analysis of transcription factor interactions in Drosophila (D. Bohmann), proteins involved in histone transport (J .Hayes), proteomic analysis ofneurodegenerative diseases (D. Pearce), Posttranslational modifications of G protein coupled receptors (P. Hinkle), comprehensive analysis of the human salivary proteome (J. Melvin), and tubulin and histone modifications in Tetrahymena (M. Gorovsky). The LC/ESI ion trap mass spectrometer will be incorporated into a functional genomics core facility that also contains a MALDI-TOF and microarray instrumentation. A Masters level mass spectrometrist will be responsible for the day to day operation of the instrument. A Mass Spectrometry Committee has been named, that includes an outside advisory member who directs a mass spectrometry core facility at Vanderbilt University that will provide long-term guidance about the use of the instrument. The institutional commitment will provide salary support for the Masters level mass spectrometrist for three (3) years and will pay the service contract for three (3) years. A cost recovery plan will be implemented that will cover costs in future years.

描述（由申请人提供）：我们要求购买用于购买蛋白质和蛋白质组分析的电喷雾离子陷阱质谱仪的资金。特定的仪器是一种热芬尼根LTQ线性四极离子陷阱质谱系统，该系统耦合到验证师HPLC系统，该系统能够100 nl到UL流速，以及纳米或电喷雾源。罗切斯特大学没有任何具有可比功能的乐器。八个主要用户和至少有两个未成年用户所证明的这项工具需要该工具，他们都是由NIH资助的。 Alan V. Smrcka（主要研究者）一直使用氢/氘交换MALDI-TOF质谱法来分析信号转导蛋白。获取该仪器将大大提高该分析的水平。 Other major users propose to use the system to study global protein modifications in yeast (E. Phizicky), proteins involved in nuclear transport of histones (J. Hayes), analysis of transcription factor interactions in Drosophila (D. Bohmann), proteins involved in histone transport (J .Hayes), proteomic analysis ofneurodegenerative diseases (D. Pearce), Posttranslational modifications of G protein偶联受体（P. hinkle），人类唾液蛋白质组（J. Melvin）的全面分析，以及小管蛋白和组蛋白修饰（M. Gorovsky）。 LC/ESI离子陷阱质谱仪将纳入功能性基因组核心设施中，该设施还包含MALDI-TOF和微阵列仪器。大师级质谱师将负责该乐器的日常运行。质谱委员会已被任命为一个质谱委员会，其中包括一个外部咨询会员，该咨询会员指挥范德比尔特大学的质谱核心设施，该设施将提供有关该工具使用的长期指导。机构承诺将为三（3）年的硕士级质谱师提供工资支持，并将支付三（3）年的服务合同。将实施一项成本恢复计划，该计划将涵盖未来几年的成本。