Zeiss LSM 510 META Confocal Microscope

描述（由申请人提供）：本提案要求支持具有最先进功能的共聚焦显微镜，用于多荧光蛋白研究，FRET成像和电生理研究。蔡司LCS 510 META共聚焦显微镜将提供目前无法获得的技术，并对11个生物医学科学独立实验室的研究工作至关重要。所有11个实验室的研究项目目前正处于关键阶段，长期、可靠和频繁地使用这些设备对进一步取得进展至关重要。所有研究人员都是由美国国立卫生研究院资助的，研究项目包括以下调查领域：离子通道和转运体功能的机制，突触发生，阿片受体的结构和功能，胚胎早期发育中的信号转导途径，心脏和血管平滑肌中的钙信号传导，毒毒碱乙酰胆碱受体的表达和定位调节，心脏梗死的干细胞修复，大麻素在神经元中的细菌发病机制和代谢及细胞作用。虽然这些实验室的研究本质上主要是基础科学，但它也对许多人类疾病和病症具有重大和直接的影响，包括心脏和血管疾病、癌症、肌肉萎缩症、细菌发病机制、视觉系统缺陷和药物成瘾和滥用。核心调查人员代表了两所学校的五个独立部门。主要用户组提出的广泛项目代表了新显微镜系统潜在用户的更大群体。所要求的仪器将放置在位于华盛顿大学校园内的凯克成像设施中，当核心研究小组不使用时，将提供给其他研究人员。介绍了该设备的运行和管理、内部咨询委员会和共聚焦显微镜的机构支持。凯克成像中心及其主任格雷格·马丁在华盛顿大学及其附属机构中享有很高的知名度和价值，因此确保了新仪器及其最先进的能力将拥有广泛的客户。

DESCRIPTION (provided by applicant): This proposal requests support for a confocal microscope with state of the art features for multi-fluorescent protein studies, FRET imaging and electrophysiological studies. The Zeiss LCS 510 META confocal microscope will provide technology that is currently unavailable to and is pivotal for the research effort of eleven independent laboratories in the biomedical sciences. Research programs in all eleven laboratories are now at a critical phase where long term, reliable, and frequent access to this equipment is essential for further progress. All investigators are NIH-funded with research programs that include the following areas of investigation: mechanisms of ion channel and transporter function, synaptogenesis, structure and function of opioid receptors, signal transduction pathways in early embryonic development, calcium signaling in cardiac and vascular smooth muscle, the regulation of expression and localization of muscarinic acetylcholine receptors, stem cell repair of cardiac infarcts, bacterial pathogenesis and metabolism and cellular actions of cannabinoids in neurons. Although the research in these laboratories is primarily basic science in nature, it also has major and direct implications for numerous human diseases and maladies, including cardiac and vascular disease, cancer, muscular dystrophy, bacterial pathogenesis, defects of the visual system and drug addiction and abuse. The core investigators represent five separate departments in two schools. The broad range of projects proposed by the primary user group is representative of a larger group of potential users of the new microscope system. The requested instrument would be placed in the established Keck Imaging Facility located on the University of Washington campus and would be made available to other investigators when it is not being used by the core group of researchers. The operation and management of this equipment, the internal advisory committee and institutional support for the confocal microscope are described. The Keck Imaging Center and its director, Greg Martin, are widely known and valued at the University of Washington and its affiliated institutions, thus assuring that the new instrument and its state-of-the- art capabilities will have a broadly based clientele.