Radiance 2100 AG-2Q Confocal Multiphoton System

描述（由申请人提供）：随着越来越多的NIH资助的研究人员使用动物模型研究疾病的分子机制和治疗，迫切需要随着时间的推移在原位、体内对组织结构和功能进行成像，而不损伤组织。活体组织的LSCM成像的主要系统相关限制是由强激光照射和激光束的有限深度穿透引起的光损伤。双光子激光扫描显微镜（TPLSM）技术克服了这些局限性。TPLSM正迅速成为研究活体动物基本生物过程的首选成像方法。我们的初步数据表明，双光子显微镜可以成功地用于中风后的活体小鼠。我们申请资金购买Radiance 2100 AG-2 Q共聚焦多光子系统，用于NIH资助的一系列赠款，包括3个P01。新系统将用于研究血栓形成、血脑屏障破坏、血管生成、轴突和树突可塑性、脑肿瘤、软骨细胞凋亡和高血压的分子和病理生理机制。免疫荧光信号和绿色荧光蛋白（GFP）信号的成像变化是每个这些国家卫生研究所资助的赠款的关键部分。Radiance 2100 AG-2 Q共聚焦多光子系统的使用将使研究人员能够大大扩展他们的拟议实验，以在不损伤或切片组织的情况下，对组织结构和功能随时间的变化进行原位成像。这将大大增加这些资助的赠款和研究在亨利福特健康科学中心的成就，以及提供一个主要的资源，研究界在底特律大都市。此外，从Radiance 2100 AG-2 Q共焦多光子系统获得的数据将为我们提出的研究机制提供新的见解，使我们能够产生新的假设，以便在资助更新和新的资助申请中进行测试。

DESCRIPTION (provided by applicant): With increasing numbers of NIH funded investigators using animal models to study molecular mechanisms and treatment of diseases, there is a compelling need to image tissue structure and function in situ, in vivo, over time without damaging tissue. The major system-related limitation of LSCM imaging of living tissues is photodamage caused by the intense laser illumination and limited depth penetration of the laser beam. Two photon laser scanning microscope (TPLSM) technology overcomes these limitations. TPLSM is fast becoming the imaging method of choice to study fundamental biological processes in living animals. Our preliminary data demonstrated that the two photon microscopy can be successfully used on the living mouse after stroke. We request funds to purchase a Radiance 2100 AG-2Q Confocal Multiphoton System to be employed in an array of NIH funded grants, including 3 P0ls. The new system will be utilized for studies of molecular and pathophysiological mechanisms underlying thrombosis, blood brain barrier disruption, angiogenesis, axonal and dendritic plasticity, brain tumor, chondrocyte apoptosis, and hypertension. Imaging changes in immunofluorescent signals and green fluorescent protein (GFP) signals are a critical part of each of these N IH funded grants. The use of Radiance 2100 AG-2Q Confocal Multiphoton System will allow investigators to greatly expand their proposed experiments to image changes in tissue structure and function in situ over time without damaging or sectioning tissue. This will greatly augment to accomplishments of these funded grants and research at Henry Ford Health Science Center as well as provide a major resource to the research community in Metropolitan Detroit. Furthermore, data obtained from the Radiance 2100 AG-2Q Confocal Multiphoton System will provide new insights into mechanisms underlying our proposed studies, permitting us to generate new hypotheses to be tested in grant renewal and new grant applications.