Radiance 2100 AG-2Q Confocal Multiphoton System

描述（由申请人提供）：随着越来越多的NIH资助的研究者使用动物模型来研究分子机制和疾病的治疗，迫切需要在不损伤组织的情况下，在原位、体内长期对组织结构和功能进行成像。活体组织LSCM成像的主要系统相关限制是由强激光照射和激光束穿透深度有限引起的光损伤。双光子激光扫描显微镜（TPLSM）技术克服了这些限制。TPLSM正迅速成为研究活体动物基本生物过程的首选成像方法。我们的初步数据表明，双光子显微镜可以成功地用于中风后的活体小鼠。我们请求资金购买Radiance 2100 AG-2Q共聚焦多光子系统，用于NIH资助的一系列拨款，包括3个项目。新系统将用于血栓形成、血脑屏障破坏、血管生成、轴突和树突可塑性、脑肿瘤、软骨细胞凋亡和高血压的分子和病理生理机制的研究。免疫荧光信号和绿色荧光蛋白（GFP）信号的成像变化是这些nih资助的赠款的关键部分。使用Radiance 2100 AG-2Q共聚焦多光子系统将允许研究人员极大地扩展他们提出的实验，在不损坏或切片组织的情况下，原位成像组织结构和功能的变化。这将大大增加亨利福特健康科学中心的资助和研究成果，并为底特律大都会的研究界提供主要资源。此外，从Radiance 2100 AG-2Q共聚焦多光子系统获得的数据将为我们拟议研究的机制提供新的见解，使我们能够产生新的假设，以便在资助续期和新的资助申请中进行测试。

DESCRIPTION (provided by applicant): With increasing numbers of NIH funded investigators using animal models to study molecular mechanisms and treatment of diseases, there is a compelling need to image tissue structure and function in situ, in vivo, over time without damaging tissue. The major system-related limitation of LSCM imaging of living tissues is photodamage caused by the intense laser illumination and limited depth penetration of the laser beam. Two photon laser scanning microscope (TPLSM) technology overcomes these limitations. TPLSM is fast becoming the imaging method of choice to study fundamental biological processes in living animals. Our preliminary data demonstrated that the two photon microscopy can be successfully used on the living mouse after stroke. We request funds to purchase a Radiance 2100 AG-2Q Confocal Multiphoton System to be employed in an array of NIH funded grants, including 3 P0ls. The new system will be utilized for studies of molecular and pathophysiological mechanisms underlying thrombosis, blood brain barrier disruption, angiogenesis, axonal and dendritic plasticity, brain tumor, chondrocyte apoptosis, and hypertension. Imaging changes in immunofluorescent signals and green fluorescent protein (GFP) signals are a critical part of each of these N IH funded grants. The use of Radiance 2100 AG-2Q Confocal Multiphoton System will allow investigators to greatly expand their proposed experiments to image changes in tissue structure and function in situ over time without damaging or sectioning tissue. This will greatly augment to accomplishments of these funded grants and research at Henry Ford Health Science Center as well as provide a major resource to the research community in Metropolitan Detroit. Furthermore, data obtained from the Radiance 2100 AG-2Q Confocal Multiphoton System will provide new insights into mechanisms underlying our proposed studies, permitting us to generate new hypotheses to be tested in grant renewal and new grant applications.