Radiance 2100 AG-2Q Confocal Multiphoton System

描述(申请人提供)：随着越来越多的NIH资助的研究人员使用动物模型来研究分子机制和疾病的治疗，随着时间的推移，迫切需要在不损害组织的情况下对组织结构和功能进行原位、活体成像。激光扫描共聚焦显微镜对活体组织成像的主要限制是强激光照射和有限深度穿透造成的光损伤。双光子激光扫描显微镜(TPLSM)技术克服了这些局限性。TPLSM正在迅速成为研究活体动物基本生物过程的成像方法。我们的初步数据表明，双光子显微镜可以成功地用于中风后的活体小鼠。我们请求资金购买Radiance 2100 AG-2Q共焦多光子系统，用于NIH资助的一系列赠款，包括3个POL。新系统将用于研究血栓形成、血脑屏障破坏、血管生成、轴突和树突可塑性、脑肿瘤、软骨细胞凋亡和高血压的分子和病理生理机制。免疫荧光信号和绿色荧光蛋白(GFP)信号的成像变化是NIH资助的每个赠款的关键部分。Radiance 2100 AG-2Q共焦多光子系统的使用将使研究人员能够极大地扩展他们提出的实验，以成像组织结构和功能随时间的原位变化，而不会损伤或切割组织。这将极大地增加亨利·福特健康科学中心这些资助拨款和研究的成果，并为底特律大都会的研究社区提供主要资源。此外，从Radiance 2100 AG-2Q共焦多光子系统获得的数据将为我们拟议研究的基础机制提供新的见解，使我们能够生成新的假设，以便在赠款续期和新的赠款申请中进行测试。

DESCRIPTION (provided by applicant): With increasing numbers of NIH funded investigators using animal models to study molecular mechanisms and treatment of diseases, there is a compelling need to image tissue structure and function in situ, in vivo, over time without damaging tissue. The major system-related limitation of LSCM imaging of living tissues is photodamage caused by the intense laser illumination and limited depth penetration of the laser beam. Two photon laser scanning microscope (TPLSM) technology overcomes these limitations. TPLSM is fast becoming the imaging method of choice to study fundamental biological processes in living animals. Our preliminary data demonstrated that the two photon microscopy can be successfully used on the living mouse after stroke. We request funds to purchase a Radiance 2100 AG-2Q Confocal Multiphoton System to be employed in an array of NIH funded grants, including 3 P0ls. The new system will be utilized for studies of molecular and pathophysiological mechanisms underlying thrombosis, blood brain barrier disruption, angiogenesis, axonal and dendritic plasticity, brain tumor, chondrocyte apoptosis, and hypertension. Imaging changes in immunofluorescent signals and green fluorescent protein (GFP) signals are a critical part of each of these N IH funded grants. The use of Radiance 2100 AG-2Q Confocal Multiphoton System will allow investigators to greatly expand their proposed experiments to image changes in tissue structure and function in situ over time without damaging or sectioning tissue. This will greatly augment to accomplishments of these funded grants and research at Henry Ford Health Science Center as well as provide a major resource to the research community in Metropolitan Detroit. Furthermore, data obtained from the Radiance 2100 AG-2Q Confocal Multiphoton System will provide new insights into mechanisms underlying our proposed studies, permitting us to generate new hypotheses to be tested in grant renewal and new grant applications.