Confocal Microscope for Core Imaging Facility

描述（申请人提供）：生物标本成像已成为生物医学科学中越来越重要的工具，对活体标本和多个荧光团成像的能力尤为重要。我们建议购买一个蔡司LSM510共聚焦显微镜系统，安置在犹他大学医学院的细胞成像设施。这种共聚焦将取代过时的入门级共聚焦（奥林巴斯FVX），并通过增加广泛的激发波长选择以及在多个发射滤波器之间的快速切换来提高设备的能力。环境控制的标本室和电动X-Y平台将特别有利于活细胞成像实验。目前在犹他大学的任何地方都没有这种功能的仪器。

DESCRIPTION (provided by applicant): Imaging of biological specimens has become an increasingly important tool in biomedical science, with the abilities to image live specimens and multiple fluorophores being especially important. We propose to purchase a Zeiss LSM510 confocal microscope system to be housed in the Cell Imaging Facility of the University of Utah School of Medicine. This confocal would replace an obsolete entry-level confocal (Olympus FVX) and improve the capabilities of the facility by adding a wide choice of excitation wavelengths, as well as fast switching between multiple emission filters. An environmentally controlled specimen chamber and motorized X-Y stage would particularly facilitate live-cell imaging experiments. No instrument with this set of features is currently available anywhere at the University of Utah. The core Cell Imaging Facility is centrally located in the School of Medicine, and is overseen by a fulltime Ph.D. director with postdoctoral experience. Under his direction the facility has been highly successful, with heavy use of the existing confocal microscopes (>35 hours/week/microscope). The director provides training and support to users in addition to maintaining the microscopes and administering their use. Furthermore, every year he teaches a didactic microscopy course together with the Principal Investigator. There is a strong institutional commitment to providing core microscopy facilities. The University of Utah fully funds the facility director's salary, and guarantees the cost of service contracts. The instrument's use would be monitored by an oversight committee, which would liaise with a committee that oversees all of the School of Medicine's core facilities. This instrument would primarily serve 7 major users from 4 different departments in the School of Medicine. These researchers are funded by 14 current R01, P01 or R37 NIH grants and are studying systems including cultured cells, C. elegans, and zebrafish. All of these projects involve live-cell imaging, and would benefit significantly from the improved flexibility in excitation and emission wavelengths offered by the proposed instrument.