ZEISS AXIOPLAN 2 IMAGING SYSTEM

描述（由申请人提供）：本修订提案是蔡司Axioplan 2成像系统的申请。该显微镜具有完整的微分干涉对比（DIG）和相位光学，暗场和明场照明，多通道荧光。成像系统包括一个高分辨率CCD相机和反褶积软件，用于去除组织切片或培养室载玻片样品中的失焦荧光。与高质量的宽视场光学相结合的反褶积已被证明可以提供比共聚焦显微镜更高分辨率的图像，并且还可以产生具有高信噪比的样品的高分辨率图像。反褶积显微镜也避免了扫描共聚焦显微镜固有的光漂白和光毒性问题。这个显微镜系统包含了广泛的功能和它的简单使用使它在日常应用中非常有用。

DE`SCRIPTION (provided by applicant): This revised proposal is a request for a Zeiss Axioplan 2 imaging system. This microscope has complete differential interference contrast (DIG) and phase optics, dark field and bright field illumination, and multichannel fluorescence. The imaging system includes a high-resolution CCD camera, and deconvolution software to remove out-of focus fluorescence from tissue sections or cultured chamber slide samples. Deconvolution in combination with the high-quality wide-field optics has been shown to provide images of greater resolution than confocal microscopes, and additionally can generate high resolution images of samples with high signal to noise ratios. Deconvolution microscopy also avoids the problems of photobleaching and phototoxicity inherent in scanning confocal microscopy. This microscope system encompasses a broad range of capabilities and its simplicity of use makes it extremely useful in everyday applications. The microscope will serve the needs of a diverse group of investigators from the Tupper Research Institute at Tufts-New England Medical Center. The broad research interests of the core user group includes the study of bacterial and protozoan pathogens, the role of transcriptional regulators in heart development, differentiation of gastrointestinal and pancreatic endocrine cells, focal adhesions in pulmonary vascular endothelial cells, the role of serotonin in vascular remodeling and pulmonary hypotension, hypoxia activation of signal transduction pathways, and the development of acute pancreatitis. The microscope will be incorporated into the Cell and Tissue Analysis Core facility of the Center for Gastroenterology Research on Absorptive and Secretory processes (GRASP) at the Tufts-New England Medical Center where it will be available to both researchers in the core user group and the broader Tufts-NEMC research community.