ZEISS AXIOPLAN 2 IMAGING SYSTEM

说明（由申请人提供）：本修订提案是对Zeiss Axioplan 2成像系统的请求。该显微镜具有完整的微分干涉差（DIG）和相位光学，暗场和亮场照明，以及多通道荧光。成像系统包括高分辨率CCD相机和去卷积软件，以从组织切片或培养的腔室载玻片样品中去除焦点外的荧光。与高质量的宽视场光学器件相结合的去卷积已经被证明可以提供比共焦显微镜更高分辨率的图像，并且还可以生成具有高信噪比的样品的高分辨率图像。去卷积显微镜还避免了扫描共聚焦显微镜中固有的光漂白和光毒性问题。该显微镜系统具有广泛的功能，其简单的使用使其在日常应用中非常有用。 该显微镜将满足塔夫茨新英格兰医学中心塔珀研究所的各种研究人员的需求。核心用户组的广泛研究兴趣包括细菌和原生动物病原体的研究，转录调节因子在心脏发育中的作用，胃肠道和胰腺内分泌细胞的分化，肺血管内皮细胞中的局灶性粘连，5-羟色胺在血管重塑和肺低血压中的作用，信号转导通路的缺氧激活以及急性胰腺炎的发展。该显微镜将被纳入Tufts-New England Medical Center的消化道吸收和分泌过程研究中心（GRASP）的细胞和组织分析核心设施，在那里它将提供给核心用户组和更广泛的Tufts-NEMC研究社区的研究人员。

DE`SCRIPTION (provided by applicant): This revised proposal is a request for a Zeiss Axioplan 2 imaging system. This microscope has complete differential interference contrast (DIG) and phase optics, dark field and bright field illumination, and multichannel fluorescence. The imaging system includes a high-resolution CCD camera, and deconvolution software to remove out-of focus fluorescence from tissue sections or cultured chamber slide samples. Deconvolution in combination with the high-quality wide-field optics has been shown to provide images of greater resolution than confocal microscopes, and additionally can generate high resolution images of samples with high signal to noise ratios. Deconvolution microscopy also avoids the problems of photobleaching and phototoxicity inherent in scanning confocal microscopy. This microscope system encompasses a broad range of capabilities and its simplicity of use makes it extremely useful in everyday applications. The microscope will serve the needs of a diverse group of investigators from the Tupper Research Institute at Tufts-New England Medical Center. The broad research interests of the core user group includes the study of bacterial and protozoan pathogens, the role of transcriptional regulators in heart development, differentiation of gastrointestinal and pancreatic endocrine cells, focal adhesions in pulmonary vascular endothelial cells, the role of serotonin in vascular remodeling and pulmonary hypotension, hypoxia activation of signal transduction pathways, and the development of acute pancreatitis. The microscope will be incorporated into the Cell and Tissue Analysis Core facility of the Center for Gastroenterology Research on Absorptive and Secretory processes (GRASP) at the Tufts-New England Medical Center where it will be available to both researchers in the core user group and the broader Tufts-NEMC research community.