Affymetrix GeneChip Scanner 3000 System

描述(由申请人提供)：我们要求一台Affymetrix基因芯片扫描仪3000系统，以完成我们在约翰霍普金斯大学医学院的最先进的微阵列核心设施。该系统是唯一一台允许处理和扫描Affymetrix新型高密度基因芯片阵列的设备，该阵列在单个微阵列中包含人类、小鼠或大鼠基因组的全面全基因组信息。该扫描仪在速度、空间最小化和微阵列扫描程序自动化方面提供了先进的设计改进。它提供了一致的扫描仪到扫描仪的整体生物学性能，提高了数据完整性，而且非常重要的是，研究人员之间一致的数据共享。本提案中包括的扫描仪附件包括一个安装了新软件和新操作系统的工作站、一个48阵列扫描仪自动加载机转盘和一个流体站，以支持在微阵列核心处理的高微阵列吞吐量。这些附件对于核心扫描仪的完全可操作性是必需的。该扫描仪将设在我们基础科学领域的中央核心设施中，并将由我们的专家和经验丰富的微阵列核心工作人员和管理人员进行监督和维护。该系统将为美国国立卫生研究院资助的大型科学社区提供服务，该社区由来自约翰·霍普金斯大学多个部门的130多名用户组成。

DESCRIPTION (provided by applicant): We request an Affymetrix GeneChip Scanner 3000 System to complete our state of the art microarray core facility at the Johns Hopkins University School of Medicine. This system is the only piece of equipment that allows the processing and scanning of Affymetrix's new high density GeneChip arrays, which contain comprehensive whole-genome information for the human, murine, or rat genomes in a single microarray. The scanner offers advanced design improvements of speed, minimization of space, and automation of microarray scanning procedures. It provides a consistent scanner-to-scanner overall biological performance, improving data integrity and very importantly, consistent data sharing between researchers. The accessories of the scanner included in this proposal are a workstation, with the new software and new operating system, a 48-array scanner autoloader carrousel, and a fluidics station to support the high microarray throughput processed at the microarray core. These accessories are needed for the full operability of the scanner at the core. This scanner will be located in our centralized core facility within the basic sciences and will be supervised and maintained by our expert and experienced microarray core staff and management. The system will serve a large NIH-funded scientific community of over 130 users from multiple departments throughout the Johns Hopkins University.