Affymetrix GeneChip Scanner 3000 System

描述（由申请人提供）：我们需要Affymetrix基因芯片扫描仪3000系统来完成我们在约翰霍普金斯大学医学院最先进的微阵列核心设施。该系统是唯一允许处理和扫描Affymetrix新型高密度基因芯片阵列的设备，该阵列包含人类，小鼠或大鼠基因组的综合全基因组信息。该扫描仪提供了先进的设计改进的速度，空间最小化，和微阵列扫描程序的自动化。它提供了一致的扫描仪到扫描仪的整体生物学性能，提高了数据的完整性，非常重要的是，研究人员之间一致的数据共享。该方案中包含的扫描仪附件包括一个工作站，带有新的软件和新的操作系统，一个48阵列扫描仪自动加载轮盘，以及一个流体站，以支持在微阵列核心处理的高微阵列吞吐量。这些附件需要在扫描仪的核心完全可操作性。该扫描仪将位于我们的基础科学集中核心设施内，并将由我们的专家和经验丰富的微阵列核心工作人员和管理层监督和维护。该系统将服务于一个由美国国立卫生研究院资助的大型科学社区，该社区有来自约翰霍普金斯大学多个部门的130多名用户。

DESCRIPTION (provided by applicant): We request an Affymetrix GeneChip Scanner 3000 System to complete our state of the art microarray core facility at the Johns Hopkins University School of Medicine. This system is the only piece of equipment that allows the processing and scanning of Affymetrix's new high density GeneChip arrays, which contain comprehensive whole-genome information for the human, murine, or rat genomes in a single microarray. The scanner offers advanced design improvements of speed, minimization of space, and automation of microarray scanning procedures. It provides a consistent scanner-to-scanner overall biological performance, improving data integrity and very importantly, consistent data sharing between researchers. The accessories of the scanner included in this proposal are a workstation, with the new software and new operating system, a 48-array scanner autoloader carrousel, and a fluidics station to support the high microarray throughput processed at the microarray core. These accessories are needed for the full operability of the scanner at the core. This scanner will be located in our centralized core facility within the basic sciences and will be supervised and maintained by our expert and experienced microarray core staff and management. The system will serve a large NIH-funded scientific community of over 130 users from multiple departments throughout the Johns Hopkins University.