Refinement of pre-exposure programs to reduce the use of fish in proliferative kidney disease monitoring in aquaculture

完善预暴露方案以减少水产养殖增殖性肾病监测中鱼类的使用

• 批准号: 2758347
• 金额: --
• 依托单位: University of Nottingham
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2022
• 资助国家: 英国
• 起止时间: 2022 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2758347
• 关键词: Refinement; pre; exposure; programs; reduce

The aim of this proposal is to optimise the Proliferative Kidney Disease (PKD) experimental challenge model to limit the use of fish as sentinels of disease in research. We propose to achieve this by adapting methods developed previously by us 1) to quantify parasite dose by water sampling and detection of environmental DNA and 2) to measure PKD specific immunity in exposed fish using serology. These methods will provide a novel best-practise challenge protocol, and a challenge monitoring toolkit. The main 3Rs advance will be to reduce the number of fish required to reach statistically acceptable sample sizes, allowing future scaling up of large field trials during vaccine development work.

本提案的目的是优化增殖性肾病（PKD）实验挑战模型，以限制在研究中使用鱼类作为疾病哨兵。我们建议采用我们以前开发的方法来实现这一目标：1)通过水采样和检测环境DNA来量化寄生虫剂量；2)使用血清学来测量暴露鱼的PKD特异性免疫。这些方法将提供一种新的最佳实践挑战协议和挑战监控工具包。3Rs的主要进展将是减少达到统计上可接受的样本量所需的鱼的数量，从而允许在疫苗开发工作期间扩大大型现场试验。