Identification of the 1-methyladenine receptor

1-甲基腺嘌呤受体的鉴定

• 批准号: 7154433
• 负责人: Sean P Place
• 金额: $ 4.6万
• 依托单位: UNIVERSITY OF CALIFORNIA SANTA BARBARA
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-01-01 至 2009-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7154433
• 关键词: Asteroidea; G protein; Xenopus oocyte; adenine analog; antisense nucleic acid; cell cycle; complementary DNA; developmental genetics; egg /ovum; expression cloning; gene expression; high throughput technology; hormone inhibitor; hormone receptor; invertebrate embryology; invertebrate hormone; meiosis; oligonucleotides; postdoctoral investigator; protein binding; protein quantitation /detection; protein structure function; yeast two hybrid system

DESCRIPTION (provided by applicant): The goal of the proposed study is to identify and characterize the oocyte maturation hormone receptor in the starfish, Patina miniata. This model system offers significant advantages over others and some aspects may be shared with mammalian oocytes. The proposed work is designed to identify and characterize this receptor using unique homology based and functional expression cloning based approaches in tandem to identify and clone candidate cDNA(s) encoding the receptor for the maturation-inducing hormone, 1-methyladenine (1- MA). Successful identification of the 1-MA receptor using these novel strategies would represent the first definitive description of a maturation-inducing hormone receptor in any species and provide proof of concept for the strategy to be used for the identification of maturation hormone receptors in other species, including mammals. Several candidate cDNAs already have been isolated; these and additional candidates will be subjected to further functional studies to confirm identity and tests for necessity in oocyte maturation will be conducted. Eventually, studies will be conducted that are designed to elucidate the details of the molecular mechanism of signal transduction during oocyte maturation.

描述（由申请人提供）：本研究的目的是鉴定和表征海星（Patina miniata）的卵母细胞成熟激素受体。该模型系统具有明显的优势，并且在某些方面可能与哺乳动物卵母细胞相同。本研究旨在利用独特的基于同源性和功能表达克隆的方法鉴定和表征该受体，同时鉴定和克隆编码成熟诱导激素1-甲基腺嘌呤（1- MA）受体的候选cDNA。使用这些新策略成功鉴定1-MA受体将代表对任何物种中成熟诱导激素受体的首次明确描述，并为将该策略用于鉴定其他物种（包括哺乳动物）的成熟激素受体提供概念证明。几个候选cdna已经被分离出来；这些和其他候选药物将进行进一步的功能研究，以确认其身份，并进行卵母细胞成熟所需的测试。最终，我们将开展旨在阐明卵母细胞成熟过程中信号转导分子机制细节的研究。