ADP-ribosylation in plant-bacteria interactions

描述(由申请人提供)：本项目将研究细菌病原体的效应蛋白如何攻击植物并导致致病结果，以及植物如何应对这些攻击。我最近在拟南芥防御反应方面的工作，实验室对丁香假单胞菌效应蛋白的分类，以及对哺乳动物细菌病原体的研究，都指出了ADP-核糖化在植物与细菌相互作用中的相关性。这是一个新的、在很大程度上未被研究的植物发病机制领域。提出了三个具体目标：1)研究最近发现的植物ADP-核糖焦磷酸酶和植物多聚ADP-核糖水解酶在植物抗病中的作用。2)植物寄主靶标的细菌ADP-核糖基化研究。将测定三种紫丁香菌ADP-核糖基转移酶效应蛋白的ADP-核糖基化活性，研究这些效应蛋白对毒力的贡献，并确定它们的植物蛋白靶标。3)研究内源多聚ADP-核糖化在植物防御和HR细胞死亡中的作用。植物病害威胁着人类的生存，是研究寄主-病原菌相互作用的信息模型。拟议的工作开辟了非常适合未来独立研究的新领域。

DESCRIPTION (provided by applicant): The present project will examine how effector proteins of bacterial pathogens attack plants and cause pathogenic outcomes, and how the plant counters these attacks. My recent work on Arabidopsis defense responses, findings from labs cataloguing Pseudomonas syringae effector proteins, and work on bacterial pathogens of mammals all point to the relevance of ADP-ribosylation in plant-bacteria interactions. This is a new, largely unstudied area of plant pathogenesis. Three Specific Aims are proposed: 1) The contributions of recently implicated plant ADP-ribose pyrophosphatase and plant poly(ADP-ribose) glycohydrolase to plant disease resistance will be examined. 2) Bacterial ADP-ribosylation of plant host targets will be studied. The ADP-ribosylation activity of three P. syringae ADP-ribosyltransferase effector proteins will be determined, the respective contributions of these effectors to virulence will be examined, and their plant protein targets will be identified. 3) The role of endogenous poly(ADP-ribosyl)ation in plant defense and HR cell death will be investigated. Plant diseases threaten human sustenance and serve as an informative model for host-pathogen interactions research. The proposed work opens new areas highly suitable for future independent research.