Genetics of reproductive isolation in Arabidopsis

描述(申请人提供)：转基因作物的转基因传播到相关的野生物种或同一作物的非转基因种植中，这在许多国家的人们中越来越受到关注。这种恐惧部分是基于对环境的担忧，也是出于对人类健康的意想不到的潜在副作用。防止不必要的转基因杂交的一个重要方法是设计对亲本植物或人类消费者不有害的遗传屏障。这将需要简单的遗传不相容。野生种群是识别对亲本菌株无害的潜在杂交障碍的一个重要且在很大程度上尚未开发的资源。我建议在这里定位和鉴定导致两个拟南芥生态型对温度敏感的杂交失活的两个基因。一旦确定了导致这些菌株不相容的基因，我将使用转基因和基因敲除方法来阐明潜在的分子机制，并测试我们是否可以在转基因杂交物中诱导出不存活的表型。并对不亲和等位基因的群体动态和分子进化进行分析，探讨它们对亲本品系是否具有适应价值。

DESCRIPTION (provided by applicant): A growing concern among people in many countries is the spread of transgenes from transgenic crop plants to related wild species or nontransgenic plantings of the same crop. This fear is based in part on environmental concerns and also for unexpected potential side effects for human health. An important method for preventing unwanted hybridizing of transgenics will be to engineer genetic barriers that are not detrimental to the parent plants or to human consumers. This would require a simple genetic incompatibility. Wild populations are one important and largely untapped resource for identifying potential hybridization barriers that are not detrimental to parent strains. I propose here to map and identify two genes that cause temperature sensitive hybrid inviability of two Arabidopsis ecotypes. Once genes underlying the incompatibility of these strains have been identified, I will use transgenic and knockout approaches to elucidate the underlying molecular mechanism, and to test the whether we can elicit the inviability phenotype in hybrids of the transgenics. I will also analyze the population dynamics and molecular evolution of the incompatibility alleles to ask whether they have adaptive value to the parental strains.