Identifying mechanisms by which neuron-oligodendrocyte interactions regulate nervous system function and dysfunction using zebrafish as a model

使用斑马鱼作为模型确定神经元-少突胶质细胞相互作用调节神经系统功能和功能障碍的机制

• 批准号: 2772544
• 金额: --
• 依托单位: University of Edinburgh
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2022
• 资助国家: 英国
• 起止时间: 2022 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2772544
• 关键词: Identifying; mechanisms; neuron; oligodendrocyte; interactions

Neural circuits, neurons interconnected by synapses, are vital for brain function and as such, it is important to establish mechanisms behind circuit formation to decipher how they are dysregulated in disease. Recent research by the Czopka lab has highlighted that the glial cell oligodendrocyte precursor cells (OPCs) play a role in regulating visual circuitry (Xiao et al., 2022). They found that OPCs regulate retinal ganglion cell (RGC) arbors, with an absence of OPCs leading to ectopic and enlarged RGCs and functional deficits in the behaviour of Zebrafish. My Phd research aims to establish how OPCs might be regulating RGC size, by investigating OPCs role in activity-dependent competition between RGC arbors and whether OPC:axon synapses stabilise RGC axons. Characterising presynapes of RGCs in the presence and absence of OPCs, silencing RGC activity, and perturbing OPC:axon synapses in the Zebrafish will help answer these questions

神经回路，即通过突触相互连接的神经元，对大脑功能至关重要，因此，重要的是要建立回路形成背后的机制，以破译它们在疾病中是如何失调的。Czopka实验室最近的研究强调了神经胶质细胞少突胶质细胞前体细胞（OPC）在调节视觉回路中起作用（Xiao et al.，2022年）。他们发现OPCs调节视网膜神经节细胞（RGC）的乔木，缺乏OPCs导致斑马鱼行为中的异位和扩大的RGC和功能缺陷。我的博士研究旨在通过研究OPC在RGC乔木之间的活性依赖性竞争中的作用以及OPC：轴突突触是否稳定RGC轴突来确定OPC如何调节RGC大小。在OPCs存在和不存在的情况下，表征RGC的突触前体，沉默RGC活性，以及干扰斑马鱼的OPC：轴突突触将有助于回答这些问题