Deciphering lipid-RNA nanocarrier structure upon RNA complexation

该项目旨在破译脂质与RNA结合后在毫秒级自组装的纳米结构演变，并跟踪生物相关酸性环境引起的纳米载体结构变化。这个项目将产生关于自组装的脂质-RNA纳米结构和细胞对象之间相互作用的新知识，以成功地释放有效载荷。该项目的预期结果是鉴定脂质-RNA分子自组装和细胞内核酸递送的基本机制。这将为RNA治疗药物的输送提供脂质纳米工程领域的重大进展。

This project aims to decipher the nanostructure evolution, at a millisecond timescale, of lipid self-assembly upon coupling with RNAs and track the nanocarrier structural changes induced by biologically relevant acidic environments. This project will generate new knowledge of the interplay between the self-assembled lipid-RNA nanostructures and cellular objects for successful payload release. The expected outcome of this project is identification of the fundamental mechanisms of lipid-RNA molecular self-assembly and intracellular nucleic acid delivery. This should provide significant advances in the field of lipid nanoparticle engineering for the delivery of RNA therapeutics.