IN VIVO EXPERIMENTATION CORE

体内实验核心

• 批准号: 6949486
• 负责人: ALEXANDER AWGULEWITSCH
• 金额: $ 13.56万
• 依托单位: MEDICAL UNIVERSITY OF SOUTH CAROLINA
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-04-01 至 2010-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6949486
• 关键词: animal colony; biomedical facility; breast neoplasms; disease /disorder model; genetically modified animals; laboratory mouse

The purpose of Core B, the In Vivo Experimentation Core (IVEC) is to provide logistical support, expertise and guidance for examining Ets gene function in vivo by using the mouse as model organism. This core consists of five components, including, (i) maintenance and husbandry of transgenic mouse strains, (ii) generation of new transgenic models, (iii) morphometrical and histological analysis of mammary gland (iv) cryopreservation, and (v) reporter gene analysis. This core structure ensures access to state-of-the-art technology to for studying mammary gland tumor development and metastasis in existing and new transgenic mouse models. Centralizing in vivo experimentation has several distinct advantages over individualized experimentation, including greater efficiency, uniformity of experimental conditions (which facilitates direct data comparison), providing a source of inspiration for developing plans or hypotheses for cross-project experimentation (e.g. crossing of mutant strains), and rapid adaptation to emerging new methodologies. Consequently, this core will be a dynamic unit with maximal responsiveness to the needs of the individual projects both at present, and as they arise during their progress.

核心B，即体内实验核心（IVEC）的目的是提供后勤支持、专业知识 对以小鼠为模型生物体内检测Ets基因功能具有指导意义。这一核心 由五个部分组成，包括：（i）转基因小鼠品系的维持和饲养，（ii） 产生新的转基因模型，（iii）乳腺的形态计量学和组织学分析（iv） 冷冻保存和（v）报告基因分析。这一核心结构确保获得最先进的 研究乳腺肿瘤在现有和新的转基因动物中的发展和转移的技术 小鼠模型。集中体内实验与个体化实验相比具有几个明显的优势。 实验，包括更高的效率，实验条件的均匀性（这有利于直接 数据比较），为制定跨项目计划或假设提供灵感来源 实验（例如突变株的杂交）和快速适应新出现的方法。 因此，这一核心将是一个充满活力的单位，能够最大限度地满足个人的需要 项目目前，以及在其进展过程中出现的。