Single Chain Antibody Core

单链抗体核心

• 批准号: 6942229
• 负责人: Bryan R. G. Williams
• 金额: $ 6.77万
• 依托单位: CLEVELAND CLINIC LERNER COM-CWRU
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-07-12 至 2010-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6942229
• 关键词: antibody; biomedical facility; immunologic substance resource /registry /referral center

This core will develop highly selective, high affinity antibodies to investigate coordinated cell signaling. Antibodies will be generated to distinguish active forms of target enzymes. The antibodies will be isolated from an established cDNA, single-chain immune library displayed on ribosomes. This library will be screened using modified peptides that mimic the effector regions of key signal proteins or, alternatively, fulllength activated proteins. This objective will be fulfilled in two specific aims: 1. To maintain immune libraries, 2. To isolate single-chain antibodies to specific antigens.

该核心将开发高选择性，高亲和力的抗体，以研究协调的细胞信号传导。 将产生抗体以区分靶酶的活性形式。抗体会被分离出来 从一个建立的cDNA，单链免疫文库展示在核糖体上。这个图书馆将是 使用模拟关键信号蛋白的效应区的修饰肽或全长肽进行筛选， 活化蛋白质这一目标将通过两个具体目标实现： 1.为了维持免疫文库， 2.分离针对特定抗原的单链抗体。