Biophysical characterisation and design of optogenetic control elements

该项目涉及最近开发的光遗传控制系统的生物物理特征，该系统基于一种天然辅酶-B12依赖蛋白。它属于“技术和方法开发”、“合成生物学”以及更广泛的“世界级生物科学”的范畴，牢牢植根于化学、生物学和物理学的交界处，这是BBSRC在“探索新的工作方式”议程中的关键驱动力。它利用数学(数据分析)的核心生物科学技能，并利用一系列溶液光谱学和质谱学技术提供重组蛋白质生产和生物物理表征方面的高级研究培训。总体而言，这项工作将为(生物)化学/生物物理学基础研究提供高度跨学科的方法，为博士生提供高度多样化的培训机会。曼彻斯特的监督员与国家物理实验室(NPL)的工业合作伙伴建立了长期的合作关系，学生将获得额外的好处，能够每天访问当地监督员的实验室，因为他们位于同一大楼(MIB)内。

The project involves the biophysical characterisation of a recently developed optogenetic control system, based on a natural coenzyme-B12 dependent protein. It falls within the remit of 'technologies and methodological development', 'synthetic biology', and more generally 'world class bioscience' and is firmly embedded at the interface of chemistry, biology and physics, a key driver for BBSRC in the 'Exploiting new ways of working' agenda. It draws on the core bioscience skills of mathematics (data analysis), and provides advanced research training in recombinant protein production and biophysical characterisation using a range of solution spectroscopy and mass spectrometry techniques. Overall, the work will provide a highly interdisciplinary approach to (bio)chemistry/ biophysics-based research, offering highly diverse training opportunities to a PhD student. The Manchester-based supervisors have long-established collaborations with the industrial partner at the National Physical Laboratory (NPL) and the student will have the additional benefit of being able to access the local supervisors' laboratories on a daily basis, as they are co-located within the same building (MIB).