PROTEOMIC

蛋白质组学

• 批准号: 7313421
• 负责人: DONALD F HUNT
• 金额: $ 8.83万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-08-10 至 2011-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7313421
• 关键词: Drosophilidae; affinity chromatography; biomedical facility; cell migration; cooperative study; functional /structural genomics; green fluorescent proteins; informatics; integrins; intermolecular interaction; mass spectrometry; phosphorylation; protein purification; protein structure function; proteins; proteomics; reporter genes

The overall goal of the Proteomics Initiative is to use mass spectrometry based proteomic methods to expand the current migration knowledge database by examining phosphorylation site utilization of proteins that regulate cell motility, by further developing methods for measuring differential phosphorylation of those proteins, and by using quantitative methods to assess positional and kinetic variation in phosphorylation site utilization in migration-related proteins. In addition, the Initiative will extend these proteomic methods to provide positional cartography of the proteins present in protein complexes formed by known migration-related proteins and protein products of novel migration genes identified in the unbiased screens of the Gene Discovery Initiative. These goals are enabled by several pivotal developments in technology, analysis, and methodology carried out during the first phase of funding. The Initiative focuses on two inter-related themes: 1. Phosphoproteomics of migration related proteins and 2. Positional proteomics of migration-related protein complexes and their phoshorylations.

蛋白质组学倡议的总体目标是使用基于质谱的蛋白质组学 通过检查磷酸化位点利用来扩展当前迁移知识数据库的方法 调节细胞运动的蛋白质，通过进一步开发用于测量 这些蛋白质，并通过使用定量方法来评估磷酸化的位置和动力学变化， 迁移相关蛋白的位点利用。此外，该倡议将把这些蛋白质组学方法扩展到 提供了存在于由已知的迁移相关的蛋白质形成的蛋白质复合物中的蛋白质的位置图 在基因的无偏筛选中鉴定的新迁移基因的蛋白质和蛋白质产物 探索计划。这些目标是由技术、分析和 在第一阶段的融资过程中。该倡议侧重于两个相互关联的主题：1. 迁移相关蛋白的磷酸化蛋白质组学; 2.迁移相关蛋白的位置蛋白质组学 配合物及其磷酸化。