Chemically modified peptide nucleic acids

化学修饰的肽核酸

• 批准号: 7152073
• 负责人: daniel appella
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7152073
• 关键词: Bacillus anthracis; amides; bacterial DNA; biohazard detection; bioterrorism /chemical warfare; microarray technology; nucleic acid chemical synthesis; nucleic acid probes; nucleic acid sequence; polynucleotides

Our research on Peptide Nucleic Acids (abbreviated as PNAs) focuses on introducing chemical modifications that will make this class of molecules broadly useful to detect sequences of DNA. Unique DNA sequences are associated with diseases, pathogens, and many agents associated with bioterrorism. Detection of DNA from these agents can be employed as a method to detect their presence or absence. Our research involves the synthesis of a class of non-natural molecules (called PNAs) that bind to specific DNA sequences. We can design our molecules to bind to any sequence of DNA, and this past year we have found that our molecules are extremely good at selective recognition of DNA associated with anthrax. Currently, we are combining our molecules with detection devices to create a robust and reliable system for detection of anthrax and other pathogens.

我们对肽核酸（缩写为 PNA）的研究重点是引入化学修饰，使此类分子广泛用于检测 DNA 序列。独特的 DNA 序列与疾病、病原体和许多与生物恐怖主义相关的因素有关。检测来自这些试剂的DNA可用作检测它们存在或不存在的方法。我们的研究涉及合成一类与特定 DNA 序列结合的非天然分子（称为 PNA）。我们可以设计我们的分子来结合任何 DNA 序列，去年我们发现我们的分子非常擅长选择性识别与炭疽相关的 DNA。目前，我们正在将我们的分子与检测设备相结合，创建一个强大而可靠的系统来检测炭疽和其他病原体。