Isolation and characterization of matrix bound vesicles for bone repair

细胞外基质（ECM）衍生的生物材料已在全球超过800万患者中使用。然而，ECM生物材料调节细胞行为的机制尚不完全清楚。最近在软组织ECM中发现的基质结合囊泡为细胞功能的调节提供了潜在的理论基础，类似于在细胞外囊泡中观察到的。该项目将使用多学科和跨领域的方法，从骨组织来源的ECM生物材料中分离和表征基质结合囊泡。该项目将从优化分离方法开始，以从骨骼中获得基质结合囊泡。然后，我们将使用OrbiSIMS、脂质组学和成像技术，通过比较细胞因子、蛋白质和代谢谱，确定分离MBV人群的病因。确定脱细胞骨mbv在细胞外生态位中的作用将有助于我们了解骨源性mbv的生物学影响。这反过来可能使骨修复新疗法的潜在发展成为可能。

Extracellular matrix (ECM) derived biomaterials have been used in more than 8 million patients worldwide. Yet, the mechanisms by which ECM biomaterials modulate cell behaviour are not fully understood. Recent discovery of matrix bound vesicles in soft tissue ECM has provided a potential rationale for regulation of cell function, analogous to that observed with extracellular vesicles.This project will use multidisciplinary and cross-cutting approaches to enable the isolation and characterisation of matrix bound vesicles from bone tissue derived ECM biomaterials. The project will commence by optimising isolation methods to obtain matrix bound vesicles from bone. We will then define the aetiology of the isolated MBV population by comparison of the cytokine, protein and metabolic profiles using OrbiSIMS, lipidomics and imaging techniques. Defining the role of decellularized bone MBVs in the extracellular niche will inform our understanding of the biological impacts of bone derived MBVs. This in turn may enable the potential development of new therapeutics for bone repair.