Animal Core

核心B是一个动物核心，将由道格拉斯A导演。库尔特，并将管理在一天一天 由玛丽·埃伦·凯利博士提供。核心还将涉及两名高级技术人员的努力： 纳尔逊和艾丽西娅白色。这个核心将产生所有的动物，用于实验中， Progam项目资助提案，包括立即处于匹鲁卡品诱导癫痫持续状态的动物 (Moss建议，项目3），动物癫痫持续状态后1-21天（所有项目），和慢性癫痫 动物，癫痫持续状态后1-3个月（AllProjects）。所有的项目都需要老鼠， 匹罗卡品诱发的癫痫持续状态所有的实验都要求老鼠在行为上或者 电监测癫痫发作活动。为了最大限度地减少 实验室，因为正确实施该癫痫模型的所有方面需要大量的 通过动物护理人员的投资，我们选择了发展一个集中的核心，负责我们所有的 动物的需要。 具体而言，动物核心将负责诱导匹鲁卡品诱导的SE、SE后护理、长期护理和长期护理。 长期视频或视频/EEC监测我们的慢性小鼠，以确认电图的存在 和/或行为性癫痫发作、插管植入和病毒及药物给药，以及所有 立体定向手术 癫痫研究的最大障碍之一是缺乏足够的特征， 准备了这种疾病的动物模型。通过结合动物模型生成的所有建议， 将项目补助金纳入一个核心设施，由动物生产和特征鉴定专家操作 癫痫模型，这应该有助于提案中的所有研究。此外，该核心将扩展 项目中的所有研究人员进行研究的能力，其中候选机制可以 操作，并评估癫痫发生的后果。长期视频/EEG监测是一种 专业，费力，困难和昂贵的事业，在该国很少有实验室能够 有能力进行这样的研究。为所有研究者提供专业的动物监测能力 使项目1、2和3中的实验能够超越蜂窝和电路表征， 实际上评估整个动物对癫痫发病率和严重程度的影响。这立即 扩大了所有这些项目的翻译影响。

Core B is an Animal Core, which will be directed by Douglas A. Coulter, and will be managed on a day to day basis by Dr. Mary Ellen Kelly. The Core will also involve the efforts of two Senior Technicians: Shareen Nelson and Alicia White. This core will generate all of the animals to be utilized in experiments in this Progam Project Grant proposal, including animals immediately in pilocarpine-induced status epilepticus (Moss proposal, Project 3), animals 1-21 days after status epilepticus (AllProjects), and chronically epileptic animals, 1-3 months after status epilepticus (AllProjects). All projects will require mice that experience pilocarpine-induced status epilepticus. and all experiments require mice to be behaviourally or electrographically monitored for seizure activity. In order to minimize potential variability between laboratories and because proper implementation of all aspects of this epilepsy model requires substantial investment by animal care personnel, we have chosen to develop a centralized core responsible for all our animal needs. Specifically the animal core will be responsible for induction of pilocarpine-induced SE, post-SE care, long- term video or video/EEC monitoring of our chronic mice in order to confirm the presence of electrographic and/or behavioural seizures, cannula implantation and administration of viruses and drugs, and all stereotaxic surgeries. One of the biggest impediments in epilepsy research is lack of adequately characterized, competently prepared animal models of this disorder. By combining animal model generation for all proposals within this Program Project Grant into one core facility, manned by experts in generation and characterization of animal models of epilepsy, this should facilitate all studies within the proposal. Additionally, this core will extend the ability of all investigators in the Program Project to conduct studies in which candidate mechanisms can be manipulated, and consequences on epileptogenesis assessed. Long-term video/EEG monitoring is a specialized, laborious, difficult and expensive undertaking, with few laboratories in the country able to competently conduct such studies. Providing this expert animal monitoring capability to all investigators enables experiments in Projects 1, 2 and 3 to move beyond cellular and circuit characterizations, and actually assess effects in whole animals, on the incidence and severity of epilepsy. This immediately expands the translational impact of all of these programs.