Morphology-Based Rating of Egg Development Potential

基于形态学的卵子发育潜力评级

• 批准号: 7019119
• 负责人: CLIFFORD C HOYT
• 金额: $ 45.61万
• 依托单位: CAMBRIDGE RESEARCH AND INSTRUMENTATION
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-02-18 至 2008-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7019119
• 关键词: biomedical automation; biomedical equipment development; biotechnology; birefringences; cell morphology; cell population study; clinical biomedical equipment; computer program /software; cryopreservation; fertility; health care cost /financing; in vitro fertilization; liquid crystal; mathematical model; meiosis; mitotic spindle apparatus; noninvasive diagnosis; oogenesis; optics; ovulation detection /prediction; reproductive development

DESCRIPTION (provided by applicant): The objective of this Phase II project is to develop a more effective tool for assessing the development potential of human oocytes and to reduce the emotional, health, and financial costs of low IVF success rates, presently approximately 25% per cycle. To compensate for these low rates, as many as three or more embryos are transferred to the mother-this practice is a leading cause of multiple and often premature births. Low success rates also lead to multiple, emotionally draining and expensive interventions. An ability to select high quality oocytes is critical to achieving successful outcomes. The main premise of this application is that reliable quantitative analysis of oocyte structures can improve IVF techniques, and proposes to apply to this task a new type of microscope that measures birefringence, an optical property exhibited in many cellular structural elements. Compared to images provided by traditional Hoffman or DIG optics presently used for oocyte assessment, non-invasive birefringence imaging using CRI's unique liquid crystal polarized light microscope dramatically and quantitatively highlights the meiotic spindle and other cellular structures by measuring birefringence retardation at each image point in the field of view. Phase I demonstrated that birefringence imaging of oocyte structures provides information that correlates significantly with embryo development and, ultimately, clinical outcome. Spindle visualization was also shown to be useful in assessing viability of cryopreserved oocytes, another critical and otherwise unsolved problem. Phase II specific aims include extending functionality to allow quantitative measurement of birefringence in three dimensions, adding features to make operation more suitable to the clinical IVF setting, developing an appropriately sized and treated glass-bottom dish, developing software tools and algorithms that extract additional quantitative information about viability and condition, and working with three beta-sites to test systems and provide vital feedback on effectiveness and operation.

描述（由申请人提供）：这个二期项目的目标是开发一种更有效的工具来评估人类卵母细胞的发育潜力，并减少低试管婴儿成功率（目前每个周期约为25%）带来的情感、健康和经济成本。为了弥补这些低比率，多达三个或更多的胚胎被移植到母亲身上——这种做法是导致多胎和经常早产的主要原因。低成功率还会导致多次、耗费情感和昂贵的干预。选择高质量卵母细胞的能力对于获得成功的结果至关重要。本应用的主要前提是对卵母细胞结构进行可靠的定量分析可以改进IVF技术，并提出将一种新型显微镜应用于这项任务，该显微镜可以测量双折射，双折射是许多细胞结构元件所表现出的光学特性。与目前用于卵母细胞评估的传统Hoffman或DIG光学提供的图像相比，使用CRI独特的液晶偏振光显微镜进行非侵入性双折射成像，通过测量视场中每个图像点的双折射延迟，显着定量地突出减数分裂纺锤体和其他细胞结构。第一阶段表明，卵母细胞结构的双折射成像提供了与胚胎发育和最终临床结果显著相关的信息。纺锤体可视化也被证明有助于评估冷冻保存的卵母细胞的活力，这是另一个关键的尚未解决的问题。第二阶段的具体目标包括扩展功能以允许三维双折射定量测量，增加功能以使操作更适合临床试管婴儿设置，开发适当大小和处理的玻璃底皿，开发软件工具和算法以提取有关生存能力和状况的额外定量信息，并与三个beta站点一起测试系统并提供有关有效性和操作的重要反馈。