Structure-based Analysis of Apocarotenoid Biosynthesis

基于结构的类胡萝卜素生物合成分析

• 批准号: 7171858
• 负责人: Michele E Auldridge
• 金额: $ 4.88万
• 依托单位: SALK INSTITUTE FOR BIOLOGICAL STUDIES
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-01-01 至 2008-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7171858
• 关键词: Abscisic Acid; Affinity Chromatography; Anabolism; Animal Experimentation; Animal Model; Animals; Architecture; Biological Assay; Carotenoids; Categories; Class; Classification; Cleaved cell; Collection; Complex; Crystallization; Data; Development; Dioxygenases; Dissection; Engineering; Enzymes; European; Family; Genes; Growth; Hormones; In Vitro; Label; Lateral; Lead; Metabolic Pathway; Mouse-ear Cress; Mutagenesis; Nutritional; Object Attachment; Oxygen; Pathway interactions; Placement; Plant Growth Regulators; Plants; Preventive; Production; Proteins; Recombinants; Research Proposals; Resolution; Retinoids; Role; Signaling Molecule; Site; Staging; Structure; Substrate Specificity; Synchrotrons; Tretinoin; Vision; Visual; Vitamin A; base; chromophore; enzyme activity; in vivo; member; novel; research study; small molecule; synchrotron radiation; three dimensional structure

DESCRIPTION (provided by applicant): Retinoids are essential signaling molecules in animals and have become common chemo-preventive dietary agents. The retinoid biosynthetic pathway begins with the cleavage of a carotenoid by a member of the Carotenoid Cleavage Dioxygenase (CCD) family of enzymes first discovered in plants. In plants, CCDs participate in the biosynthesis of the phytohormone abscisic acid and a novel hormone that controls plant architecture. In animals the CCDs are responsible for the biosynthesis of retinoids, notably vitamin A, which is necessary for vision, and retinoic acid, which is critical to normal development. Currently, no high resolution structural information for any CCD exists. With the structure of one or more CCDs, a structurally guided approach towards enzyme and metabolic pathway engineering in plants could be done to allow for the control of plant architecture as well as increase nutritional quality. In animals, this approach could lead to a greater understanding of the production of the visual chromophore and to the signaling molecule, retinoic acid. The CCD family in the model organism Arabidopsis thaliana provides a collection of related genes for the systematic dissection of the structural and functional features of the mechanistically complex CCDs.

描述（由申请人提供）：类视黄醇是动物体内​​必需的信号分子，并已成为常见的化学预防饮食剂。 类视黄醇生物合成途径始于类胡萝卜素被首先在植物中发现的类胡萝卜素裂解双加氧酶 (CCD) 家族成员的裂解。 在植物中，CCD 参与植物激素脱落酸和控制植物结构的新型激素的生物合成。 在动物中，CCD 负责类视黄醇的生物合成，尤其是视力必需的维生素 A 和对正常发育至关重要的视黄酸。 目前，任何 CCD 都不存在高分辨率结构信息。 利用一个或多个 CCD 的结构，可以对植物中的酶和代谢途径工程进行结构引导的方法，以控制植物结构并提高营养质量。 在动物中，这种方法可以使人们更好地了解视觉发色团的产生和信号分子视黄酸。 模式生物拟南芥中的 CCD 家族提供了一系列相关基因，用于系统剖析机械复杂的 CCD 的结构和功能特征。