Myosin Vb involvement in plasma membrane recycling

肌球蛋白 Vb 参与质膜回收

• 批准号: 7288264
• 负责人: JOSEPH Thomas Edward ROLAND
• 金额: $ 4.83万
• 依托单位: VANDERBILT UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-12-01 至 2008-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7288264
• 关键词: Apical; Back; Binding; Cell membrane; Cells; Complement; Complex; Endocytosis; Environment; Epithelial Cells; Family; Gastric Parietal Cells; H(+)-K(+)-Exchanging ATPase; Human; Ion Channel; Ion Pumps; Lateral; Mass Spectrum Analysis; Membrane; Molecular Motors; Monomeric GTP-Binding Proteins; Motor; Myosin ATPase; Play; Protein Array; Proteins; Recycling; Regulation; Role; Screening procedure; Surface; System; Vesicle; Work; Yeasts; base; member; polarized cell; rab11 protein; receptor; trafficking; yeast two hybrid system

Polarized epithelial cells are a dramatic example of the importance of the organized transport of cellular components. These cells must interact with two distinct environments, one on their apical surface and one on their basal/lateral surfaces. Previous work in the Goldenring lab has shown that the complex of the small GTPase Rab11a and the unconventional molecular motor myosin Vb are important in the apical transport of plasma membrane recycling vesicles in polarized epithelial cells. A combination of specific and random mutational analysis complemented by yeast two-hybrid screening will be utilized in order to determine the structural basis of the interaction between myosin Vb and its binding partners Rab11a and Rab11-FIP2. Additionaly, the components of the endogenous myosin Vb motor complex will be isolated from human gastric parietal cells by immunoprecipitaion of the motor from H/K-ATPase-enriched tubulovesicles, and the identities of the members of the complex will be determined by mass spectroscopy. Results from this work will aid in discovering the mechanisms of regulation of myosin Vb, which in turn will advance our understanding of the general mechanisms of membrane recycling in polarized cells.

极化上皮细胞是细胞有组织运输重要性的一个戏剧性例子