The Role of IME4 in cell fate determination

IME4 在细胞命运决定中的作用

• 批准号: 7235275
• 负责人: CINTIA Fabiana HONGAY
• 金额: $ 5.04万
• 依托单位: WHITEHEAD INSTITUTE FOR BIOMEDICAL RES
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-07-01 至 2008-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7235275
• 关键词: Agar; Animal Model; Antisense RNA; Binding Sites; Cancer Cell Growth; Cancer Patient; Candida albicans; Cells; Development; Diploid Cells; Filament; Genes; Goals; Growth; Haploidy; Homologous Gene; Human; Hyphae; Immunocompromised Host; Individual; Infection; Invasive; Laboratories; Left; Life; Link; Mating Types; Meiosis; Modeling; Molecular; Morphogenesis; Mus; Nucleic Acid Regulatory Sequences; Pathogenicity; Pathway interactions; Play; RNA; Range; Regulation; Role; Saccharomyces cerevisiae; Signal Pathway; Systemic infection; Transcript; Virulence; Work; Yeasts; chemotherapy; mortality; pathogen; research study

DESCRIPTION (provided by applicant): Objective/Hypothesis: Recent work from Dr. Fink's laboratory suggests that IME4 (early inducer of meiosis gene) acts as a switch in determining whether cells will undergo meiosis or filament and produces an antisense transcript under a/alpha control. Because of the connection between IME4 and the filamentation pathway in S. cerevisiae, and since there is both an IME4 homolog and MTL a1/alpha2 binding sites in the gene regulatory regions of C. albicans' IME4, my hypothesis is that a1/alpha2 plays a role in regulating IME4 antisense and sense expression and that IME4 regulation in S. cerevisiae is conserved in C. albicans. This regulatory mechanism is likely to have potential implications in the C. albicans' pathogenically important morphological switch from yeast to filamentous growth. Specific Aims: I. Determine the role and regulatory mechanism of IME4 in cell-fate determination in S. cerevisiae. II. Elucidate the function and mechanism of regulation of C. albicans' IME4.

描述（由申请人提供）：目的/假设：Fink博士实验室的最新研究表明，IME 4（减数分裂基因的早期诱导物）在决定细胞是否将进行减数分裂或丝状分裂中起开关作用，并在α/α控制下产生反义转录物。由于IME 4与S.由于C. cerevisiae的基因调控区中既有IME 4同源物又有MTL a1/alpha 2结合位点，因此C.白念珠菌的IME 4，我们推测α 1/α 2在调控IME 4的反义和正义表达中起作用，IME 4在S. cerevisiae在C.白色念珠菌这种调节机制可能对C.白色念珠菌从酵母菌到丝状生长的重要形态学转变。具体目标：一。确定IME 4在S.啤酒。 二.阐明C. albicans'IME 4.