Rigaku/MSC High-Throughput HomeLab x-ray crystallography system

描述(由申请人提供)：弗雷德·哈钦森癌症研究中心的结构分子生物学项目寻求对我们共享的内部X射线结晶学设施进行重大升级，以保持其存在十多年来所取得的高质量研究和生产力水平。这次升级将用最先进的系统(Rigaku/MSC High-Throughput HomeLab)取代老化的设备，最大限度地利用现有设施中仍然有用的设备。此次升级解决了3个主要问题：1)更换了即将失去制造商支持的失效(12年以上)图像板探测器(R轴Me)；2)极大地扩展了当前系统的能力，允许进行以前需要使用我们有限的同步加速器时间的内部实验；3)增加了内部数据收集吞吐量，以缓解目前饱和的设施的等待时间，并促进主要用户的个人研究计划在不久的将来大幅扩展。Hutchinson的结构分子生物学计划包括3个主要用户(Ferre-D‘Amare、Stoddard、Strong)，同时还支持3个次要用户(Cronk、Hockenberg、McFarland)，其中2人是前Hutchinson博士后，他们在没有X射线设备的本科机构开始独立工作。个别研究计划是多样的，但互补性很强，而且完全整合，在结构酶学(Ferre-D‘Amare，Cronk，Stoddard)、结构分子免疫学(McFarland，Strong)、核酶功能和RNA拓扑(Ferre-D’Amare)、内含子编码的限制性内切酶(Stoddard)、细胞凋亡(Hockenberg)、基于结构的药物设计(Hockenberg，Stoddard)以及蛋白质设计和结构分析(Stoddard，Strong)新方法的开创性开发等领域产生了重大影响。该项目还支持非常成功的博士后和博士后培训项目，与4个独立的研究生项目(分子与细胞生物学(FHCRC/UW)、生物化学(UW)、生物分子结构与设计(UW)和免疫学(UW)相结合)。研究和培训计划的深度和广度不断增长，越来越需要升级的X射线仪器，这对于防止生产力的严重损失是必要的，因为我们的2个图像板探测器中的1个(不受支持的R轴IIc)可能会因不可修复的故障而最终损失。

DESCRIPTION (provided by applicant): The Structural Molecular Biology program of the Fred Hutchinson Cancer Research Center seeks a major upgrade of our shared, in-house x-ray crystallography facility in order to maintain the high quality of research and level of productivity achieved over the past decade-plus of its existence. This upgrade would replace aging equipment with a state-of-the-art system (a Rigaku/MSC High-Throughput HomeLab), maximally leveraging still useful equipment in the current facility. This upgrade addresses 3 major concerns: 1) Replacement of a failing (12+ years) image plate detector (R-AXIS Me) that is soon to lose manufacturer support; 2) Dramatically expanding the capability of the current system to allow in-house experiments previously requiring use of our limited synchrotron time; 3) Increasing the in-house data collection throughput to relieve wait-times on a currently saturated facility and facilitate dramatic expansions of the individual research programs of the Major Users in the near future. The Hutchinson's Structural Molecular Biology program comprises 3 major users (Ferre-D'Amare, Stoddard, Strong) while also supporting 3 minor users (Cronk, Hockenbery, McFarland), 2 of whom are ex-Hutchinson post-docs that have started independent positions at undergraduate institutions lacking x-ray equipment. The individual research programs are diverse, but highly complementary and fully integrated, generating high-impact results in the fields of structural enzymology (Ferre-D'Amare, Cronk, Stoddard), structural molecular immunology (McFarland, Strong), ribozyme function and RNA topology (Ferre-D'Amare), intron-encoded restriction endonucleases (Stoddard), apoptosis (Hockenbery), structure-based drug design (Hockenbery, Stoddard) and pioneering the development of new methods in protein design and structure analysis (Stoddard, Strong). The program also supports a highly successful pre-and post-doctoral training program, interfacing with 4 separate graduate programs (Molecular and Cellular Biology (FHCRC/UW), Biochemistry (UW), Biomolecular Structure & Design (UW) and Immunology (UW)). The growing depth and breadth of the research and training programs increasingly require upgraded x-ray instrumentation and are necessary to prevent a crippling loss in productivity with the anticipated, eventual loss, due to an unrepairable breakdown, of 1 of our 2 image plate detectors (the unsupported R-AXIS IIc).