JOEL MODEL JEM-1230 TRANSMISSION ELECTRON MICROSCOPE

描述(由申请人提供)： 请拨资金支持购买新的JEOL透射式电子显微镜，JEM-1230型，配有AMT-X100高分辨率数码相机系统。这台显微镜将由医学院(SOM)内的高级显微镜设施(AMF)管理和维护。一个由8名高级教员和AMF技术总监组成的咨询委员会将监督该仪器。主要调查者是AMF学院主任兼咨询委员会主席。AMF是整个医学院用户的核心收费服务设施。每年约有72名首席调查人员和他们的研究小组使用这些设施。目前，AMF运营着两台电子显微镜，每台都有20多年的历史，不再可靠。在SOM和校园其他地方还有几个其他的电子显微镜，但它们是在单独的实验室里，NIH资助的研究人员通常不能获得。JEM-1230型透射电子显微镜将填补弗吉尼亚大学对可靠、高分辨率、高对比度透射电子显微镜的关键需求。这在AMF的多用户设置中将特别有价值，因为用户可以存储每个人的个性化设置。改进后的成像功能将对几个使用旋转阴影来确定蛋白质复合体的整体构象的项目以及对光束损伤敏感的脆弱样本具有重要意义。显微镜将被安置在新装修的AMF专用空间内。高级官员会议还为医疗保险基金的技术主任和一名实验室专家的工资提供支持。将有5个由NIH资助的主要用户，以及至少4个次要用户。仪器时间将由用户在基于Web的日历上保留。每小时的费用和日程安排将由咨询委员会和PI确定。

DESCRIPTION (provided by applicant): Funds are requested to support purchase of a new JEOL transmission electron microscope, model JEM- 1230, with an AMT-X100 high-resolution digital camera system. This microscope will be administered and maintained by the Advanced Microscopy Facility (AMF) within the School of Medicine (SOM). An advisory committee, consisting of 8 senior faculty plus the Technical Director of the AMF, will oversee the instrument. The principal investigator is the AMF Faculty Director and chair of the advisory committee. The AMF is a core fee-for service facility for users throughout the Medical School. About 72 principal investigators and their research groups use these facilities each year. Currently the AMF operates two electron microscopes that each are more than 20 years old, and are no longer reliable. There are several other electron microscopes within the SOM and elsewhere on campus, but they are in individual laboratories and are not generally available to NIH-funded investigators. The JEM-1230 TEM will fill a critical need within the University of Virginia for reliable, high resolution, high contrast transmission electron microscopy. It will be particularly valuable in the multi-user setting of the AMF because users can each store personalized settings. The improved imaging features will be important for several projects that use rotary shadowing to determine the overall conformation of protein complexes, and for fragile specimens that are sensitive to beam damage. The microscope will be housed in newly renovated space dedicated to the AMF. The SOM also supports the salaries of the Technical Director and a laboratory specialist in the AMF. There will be 5 NIH-funded primary users, and at least 4 minor users. Instrument time will be reserved by users on a Web-based calendar. The hourly fee and scheduling will be determined by the advisory committee and PI.