Laser Scanning Confocal Microscope

描述（由申请人提供）：申请购买蔡司LSM510激光共聚焦显微镜。有一个强有力的理由，新的共聚焦激光显微镜，以取代我们现有的大部分过时的模型，目前服务于所有美国国立卫生研究院资助的研究人员在我们的研究所。新显微镜将扩展我们成像设备的能力，同时为7名申请研究者及其合作研究者提供急需的和有价值的研究工具，他们构成了主要的用户群体。在NKI附近找不到与所要求的仪器相媲美的仪器。通过最好的技术手段，包括激光共聚焦显微镜，对结构和细胞生物学数据的分析和解释，对正在进行的研究项目的继续和加强，以及这些项目在新方向上的发展至关重要。获得新仪器将使我们研究所的科学家能够围绕神经退行性疾病和神经精神疾病的基础和转化神经科学主题探索各种主题。申请中引用的所有nih资助的项目都有需要并将通过共聚焦激光显微镜加强的组件。总结如下：1)动物模型治疗干预后ad相关蛋白的表达；2)神经退行性疾病的表达谱分析；3)半胱抑素C在脑出血和阿尔茨海默病（AD）中的作用；4) AD和唐氏综合征的内吞途径；5) AD发病机制中的内体-自噬-溶酶体系统；6)胆固醇代谢紊乱在AD发病中的作用；7)神经精神疾病的信号转导。

DESCRIPTION (provided by applicant): Funds are requested for the purchase of a Zeiss LSM510 laser confocal microscope. There is a strong justification for a new confocal laser microscope to replace our existing largely outdated model that currently serves all of the NIH-funded researchers at our Institute. The new microscope will extend the capabilities of our imaging facility, while providing a much-needed and valuable research tool for the seven applicant investigators and their co-investigators, which form the major user group. Instrumentation comparable to that requested is not available near NKI. The analysis and interpretation of structural and cell biological data by the best technological means available, including laser confocal microscopy, is of critical importance to the continuation and enhancement of ongoing research programs, and to the evolution of these programs in new directions. Acquisition of the new instrument will enable scientists throughout our institute to explore a diversity of topics organized around the theme of basic and translational neuroscience of neurodegenerative and neuropsychiatric diseases. All of the NIH-funded programs referenced in the application have components that require and would be strengthened by confocal laser microscopy. These are summarized as follows: 1) AD-related proteins following therapeutic intervention in animal models; 2) Expression Profiling in Neurodegenerative Disorders; 3) Cystatin C in cerebral hemorrhage and in Alzheimer's disease (AD); 4) The endocytic pathway in AD and Down's Syndrome; 5) The endosomal-autophagic-lysosomal system in AD Pathogenesis; 6) The role of disrupted cholesterol metabolism in AD pathogenesis; and 7) Signal Transduction in Neuropsychiatric Disease. An enhancement of the Institute's imaging capabilities will allow all applicant investigators to pursue funded research programs with greater efficiency and depth, to expand existing programs in new directions and to develop new programs.