Laser Scanning Confocal Microscope

描述(由申请人提供)：申请资金用于购买蔡司LSM510激光共聚焦显微镜。有一个新的共聚焦激光显微镜有充分的理由来取代我们现有的基本上过时的模型，该模型目前服务于我们研究所所有由NIH资助的研究人员。新的显微镜将扩展我们成像设施的能力，同时为构成主要用户群体的七名申请调查人员及其合作调查人员提供亟需的有价值的研究工具。NKI附近没有可与所要求的仪器相媲美的仪器。利用现有的最佳技术手段，包括激光共聚焦显微镜，对结构和细胞生物学数据进行分析和解释，对于继续和加强正在进行的研究计划，以及这些计划向新的方向发展至关重要。购买新仪器将使我们研究所的科学家能够探索围绕神经退行性疾病和神经精神疾病的基础神经科学和翻译神经科学这一主题组织的各种主题。申请中提到的所有NIH资助的项目都有需要并将通过共聚焦激光显微镜进行强化的组件。综述如下：1)动物模型治疗干预后的AD相关蛋白；2)神经退行性疾病中的表达谱；3)Cystatin C在脑出血和阿尔茨海默病中的作用；4)AD和唐氏综合征中的细胞内途径；5)AD发病机制中的内体-自噬-溶酶体系统；6)胆固醇代谢紊乱在AD发病中的作用；以及7)神经精神疾病中的信号转导。 该研究所成像能力的增强将使所有申请研究人员能够以更高的效率和深度进行资助的研究项目，向新的方向扩展现有的项目，并开发新的项目。

DESCRIPTION (provided by applicant): Funds are requested for the purchase of a Zeiss LSM510 laser confocal microscope. There is a strong justification for a new confocal laser microscope to replace our existing largely outdated model that currently serves all of the NIH-funded researchers at our Institute. The new microscope will extend the capabilities of our imaging facility, while providing a much-needed and valuable research tool for the seven applicant investigators and their co-investigators, which form the major user group. Instrumentation comparable to that requested is not available near NKI. The analysis and interpretation of structural and cell biological data by the best technological means available, including laser confocal microscopy, is of critical importance to the continuation and enhancement of ongoing research programs, and to the evolution of these programs in new directions. Acquisition of the new instrument will enable scientists throughout our institute to explore a diversity of topics organized around the theme of basic and translational neuroscience of neurodegenerative and neuropsychiatric diseases. All of the NIH-funded programs referenced in the application have components that require and would be strengthened by confocal laser microscopy. These are summarized as follows: 1) AD-related proteins following therapeutic intervention in animal models; 2) Expression Profiling in Neurodegenerative Disorders; 3) Cystatin C in cerebral hemorrhage and in Alzheimer's disease (AD); 4) The endocytic pathway in AD and Down's Syndrome; 5) The endosomal-autophagic-lysosomal system in AD Pathogenesis; 6) The role of disrupted cholesterol metabolism in AD pathogenesis; and 7) Signal Transduction in Neuropsychiatric Disease. An enhancement of the Institute's imaging capabilities will allow all applicant investigators to pursue funded research programs with greater efficiency and depth, to expand existing programs in new directions and to develop new programs.