Laser Scanning Confocal Microscope

描述（由申请人提供）：资金要求购买蔡司LSM 510激光共聚焦显微镜。有一个新的共聚焦激光显微镜，以取代我们现有的基本上过时的模型，目前服务于所有的NIH资助的研究人员在我们的研究所强有力的理由。新的显微镜将扩展我们的成像设备的能力，同时为七名申请研究人员及其共同研究人员提供急需的和有价值的研究工具，这些研究人员构成了主要的用户群。NKI附近没有与所要求的仪器相当的仪器。通过现有的最佳技术手段（包括激光共聚焦显微镜）对结构和细胞生物学数据进行分析和解释，对于继续和加强正在进行的研究计划以及这些计划向新方向发展至关重要。新仪器的获得将使我们整个研究所的科学家能够探索围绕神经退行性疾病和神经精神疾病的基础和转化神经科学主题组织的各种主题。申请中提到的所有NIH资助的项目都有需要共聚焦激光显微镜的组件，并将通过共聚焦激光显微镜得到加强。这些总结如下：1）动物模型中治疗干预后的AD相关蛋白; 2）神经退行性疾病中的表达谱; 3）脑出血和阿尔茨海默病（AD）中的胱抑素C; 4）AD和唐氏综合征中的内吞途径; 5）AD发病机制中的内体-自噬-溶酶体系统; 6）胆固醇代谢紊乱在AD发病机制中的作用;和7）神经精神疾病中的信号转导。 该研究所成像能力的增强将使所有申请研究人员能够以更高的效率和深度开展受资助的研究项目，在新的方向上扩大现有项目，并开发新的项目。

DESCRIPTION (provided by applicant): Funds are requested for the purchase of a Zeiss LSM510 laser confocal microscope. There is a strong justification for a new confocal laser microscope to replace our existing largely outdated model that currently serves all of the NIH-funded researchers at our Institute. The new microscope will extend the capabilities of our imaging facility, while providing a much-needed and valuable research tool for the seven applicant investigators and their co-investigators, which form the major user group. Instrumentation comparable to that requested is not available near NKI. The analysis and interpretation of structural and cell biological data by the best technological means available, including laser confocal microscopy, is of critical importance to the continuation and enhancement of ongoing research programs, and to the evolution of these programs in new directions. Acquisition of the new instrument will enable scientists throughout our institute to explore a diversity of topics organized around the theme of basic and translational neuroscience of neurodegenerative and neuropsychiatric diseases. All of the NIH-funded programs referenced in the application have components that require and would be strengthened by confocal laser microscopy. These are summarized as follows: 1) AD-related proteins following therapeutic intervention in animal models; 2) Expression Profiling in Neurodegenerative Disorders; 3) Cystatin C in cerebral hemorrhage and in Alzheimer's disease (AD); 4) The endocytic pathway in AD and Down's Syndrome; 5) The endosomal-autophagic-lysosomal system in AD Pathogenesis; 6) The role of disrupted cholesterol metabolism in AD pathogenesis; and 7) Signal Transduction in Neuropsychiatric Disease. An enhancement of the Institute's imaging capabilities will allow all applicant investigators to pursue funded research programs with greater efficiency and depth, to expand existing programs in new directions and to develop new programs.