Zeiss Axiovert 200-M for Time-Lapse Microscopy

描述（由申请人提供）：这是一个蔡司倒置显微镜的应用，配有精密电动载物台和灵敏的CCD相机，用于延时活细胞成像。该显微镜将被用作一个专用站，用于恒定焦点延时成像与DIG和荧光光学。精密的AS）电动压电显微镜载物台和蔡司环境控制系统将使我们能够在生理条件下连续几小时或几天对细胞进行成像。该阶段还将用于在同一成像会话期间准备不同位置处的细胞的延时序列。灵敏的Photometrics Cascade CCD相机将显著减少光漂白并保持细胞活力。该应用程序基于通过延时记录随时间推移监测细胞反应的关键需求，并将服务于大型NIH资助的社区。

DESCRIPTION (provided by applicant): This is an application for a Zeiss inverted microscope with precision motorized stage and sensitive CCD camera for time-lapse, live-cell imaging. The microscope will be used as a dedicated station for constant-focus time-lapse imaging with DIG and fluorescence optics. The precision AS) motorized piezo microscope stage and the Zeiss environmental control system will allow us to image cells at constant focus under physiological conditions for hours or days at a time. This stage will also be used to prepare time-lapse sequences of cells at different locations during the same imaging session. The sensitive Photometrics Cascade CCD camera will significantly reduce photobleaching and maintain cell viability. This application is based on a critical need to monitor cell responses over time by time-lapse recording and will serve a large NIH-funded community.