Zeiss LSM510 Confocal Microscope

蔡司 LSM510 共焦显微镜

• 批准号: 6877345
• 负责人: ERIC A SHELDEN
• 金额: $ 46.06万
• 依托单位: WASHINGTON STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-04-01 至 2006-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6877345
• 关键词: biomedical equipment purchase; confocal scanning microscopy; fluorescence resonance energy transfer; fluorescent dye /probe; green fluorescent proteins; molecular /cellular imaging; protein protein interaction

DESCRIPTION (provided by applicant): A group of 10 NIH funded investigators request funds to purchase a laser scanning confocal microscope. The requested system consists of a Zeiss LSM510 equipped for Visible/Near UV imaging. The group is located in two departments at the Washington State University with research ties to the neighboring University of Idaho. 79 NIH funded investigators with a total of 102 active NIH grants are located in the region. The group shares access with the general research community to a BIORAD MRC1024 now in its seventh year of heavy use. 24 laboratories used the instrument in 2003, and newly recruited investigators with research emphasizing confocal imaging will add to the demand for this instrument beginning in 2004. In addition to lack of sufficient access for long-term time-lapse studies, the group is severely limited by technical requirements that cannot be met with the existing instrument. Among these are the ability to image more than two fluorescent probes simultaneously, increased detector sensitivity permitting study of living specimens and photolabile structure, the ability to detect UV excitable probes and the ability to assess protein-protein interaction using fluorescence resonance energy transfer (FRET) measurements. The user group includes investigators with extensive previous experience with the Zeiss LSM510 confocal microscope system at other institutions, and there is no question regarding its ability to serve the needs of our user group for the life-span of the instrument. The instrument will be housed in an established multiuser imaging facility known as the "EM center" which currently offers support by two full time staff members. It is anticipated that the user group requesting the instrument will use the instrument 75% of the time, but provisions will be made for use by non-group members as time permits. Policy for the instrument will be determined by an internal advisory committee, and day-to-day maintenance, technical supervision and instruction will be provided by staff members of the EM center. Service contracts over the next 5 years will be covered by a recharge system and through financial support from the Department of Veterinary, Comparative Anatomy, Pharmacology and Physiology. As the instrument becomes integrated into the user group's ongoing research programs, it is expected that service agreements will be maintained beyond the initial 5 year period by user fees.

描述（由申请人提供）：一组由10名NIH资助的研究人员要求资金购买激光扫描共聚焦显微镜。所要求的系统由配备用于可见光/近紫外成像的Zeiss LSM 510组成。该小组位于华盛顿州立大学的两个系，与邻近的爱达荷州大学有研究联系。该地区有79名NIH资助的研究人员，共有102名NIH赠款的研究人员。该小组与一般研究团体共享BIORAD MRC 1024的使用权，目前已进入其大量使用的第七个年头。2003年有24个实验室使用了该仪器，从2004年开始，新招募的研究人员的研究重点是共聚焦成像，这将增加对该仪器的需求。除了缺乏足够的机会进行长期的延时研究外，该小组还受到现有仪器无法满足的技术要求的严重限制。其中包括能够同时成像两个以上的荧光探针，增加检测器的灵敏度，允许研究活标本和光不稳定的结构，检测UV可激发探针的能力和使用荧光共振能量转移（FRET）测量评估蛋白质-蛋白质相互作用的能力。用户组包括在其他机构使用蔡司LSM 510共聚焦显微镜系统的丰富经验的研究人员，并且毫无疑问，它能够满足我们用户组对仪器寿命的需求。该仪器将被安置在一个已建立的多用户成像设施，称为“EM中心”，目前提供支持的两个全职工作人员。预计申请该仪器的用户组将在75%的时间内使用该仪器，但将在时间允许的情况下为非用户组成员提供使用。仪器的政策将由内部咨询委员会确定，EM中心的工作人员将提供日常维护、技术监督和指导。未来5年的服务合同将由一个充值系统和兽医、比较解剖学、药理学和生理学系的财政支持支付。随着该仪器被纳入用户组正在进行的研究计划，预计服务协议将通过用户费用维持超过最初的5年期限。