TSE Inhalation System

描述（由申请人提供）：尼古丁和多种致癌物已知会导致烟草成瘾和对健康的不良影响。然而，烟草许多不利影响的致病因素尚未查明，其他烟草或烟草烟雾成分可能起作用。识别和研究这些额外的化合物对于了解与烟草有关的疾病和成瘾的机制以及评估目前正在销售的新型改性卷烟和其他烟草制品的风险具有重要意义。这些问题是明尼苏达大学（U of MN） NIDA/ nci资助的跨学科烟草研究中心（TTURC）的一个具体重点，所要求的仪器将大大扩大该中心的工作范围。本提案的具体目的是获得一种产生香烟烟雾的TSE吸入系统，该系统可用于实验动物直接暴露于烟草烟雾中，用于制备用于体外使用的烟草凝聚物，以及用于香烟烟雾中单个成分的定量。研究项目将包括（项目1，研究员Hecht）癌症化学预防和致癌机制，（项目2，研究员Murphy）尼古丁代谢，（项目3，研究员Pentel）成瘾相关行为和妊娠期吸烟暴露的影响，（项目4，研究员Peterson）神经干细胞分化，（项目5，研究员Ondrey）上气消化癌，以及（项目6，研究员hercht）。研究者Bechtold)骨愈合和种植体整合。TSE吸入系统由自动香烟烟雾发生器、混合和稀释室、烟雾监测仪器和小动物暴露室组成。烟雾特性和代表性烟雾成分可以实时监测和控制。具有烟草化学和分析技术经验的核心调查人员（Hecht, Murphy, Pentel）将协助其他调查人员获得和表征烟草提取物以及烟草成分暴露的生物测量。TTURC的核心分析实验室将为这些分析提供便利。TSE系统将被安置在距离明尼苏达大学5分钟路程的MMRF，它将提供空间和所需的设施修改。Pentel实验室将由一名研究助理监督其操作，该助理将为其他实验室提供服务，包括进行动物接触实验，并制备烟草凝聚物，然后根据各个实验室的需要进行进一步加工和表征。这种工具将大大扩展所涉及的具体项目的能力和范围。

DESCRIPTION (provided by applicant): Nicotine and a variety of carcinogens are known to contribute to the addictive and adverse health effects of tobacco use. However, the causative agents for many adverse effects of tobacco have not been identified, and additional tobacco or tobacco smoke constituents may contribute. Identifying and studying these additional compounds is important for understanding the mechanisms of tobacco-related disease and addiction, and for assessing the risks of newer modified cigarettes and other tobacco products that are now being marketed. These questions are a specific focus of the University of Minnesota (U of MN) NIDA/NCI-funded Transdisciplinary Tobacco Research Center (TTURC), and the requested instrumentation will substantially expand the scope of the Center's work. The Specific Aim of this proposal is to obtain a TSE Inhalation System to generate cigarette smoke which can be used for the direct exposure of experimental animals to tobacco smoke, for preparation of tobacco condensates for in vitro use, and for quantitation of individual constituents of cigarette smoke. Research projects will include (Project number 1, investigator Hecht) cancer chemoprevention and mechanisms of carcinogenesis, (Project number 2, investigator Murphy) nicotine metabolism, (Project number 3, investigator Pentel) addiction-related behaviors and effects of gestational smoke exposure, (Project number 4, investigator Peterson) neural stem cell differentiation, (Project number 5, investigator Ondrey) upper aerodigestive carcinogenesis, and (Project number 6, investigator Bechtold) bone healing and implant integration. The TSE Inhalation System consists of an automated cigarette smoke generator, mixing and diluting chambers, smoke monitoring instrumentation, and small animal exposure chambers. Smoke characteristics and representative smoke constituents can be monitored and controlled in real time. A core of investigators (Hecht, Murphy, Pentel) with experience in tobacco chemistry and analytical techniques will assist other investigators in obtaining and characterizing tobacco extracts and biological measures of tobacco constituent exposure. The Core Analytical Laboratory of the TTURC will be made available to facilitate these analyses. The TSE system will be housed at MMRF, located 5 minutes from the U of MN, which will provide space and the required facilities modifications. The Pentel laboratory will supervise its operation by a single research assistant who will provide services for the other laboratories, including performing animal exposure experiments, and preparing tobacco condensates which can then be further processed and characterized as needed by the individual laboratories. This instrumentation will considerably expand the capabilities and scope of the specific projects involved.