Elucidating the role of a novel virus-induced nuclear condensate in non-coding RNA biogenesis and the importance of the royal family of m6A reader pro

阐明新型病毒诱导的核凝聚物在非编码RNA生物发生中的作用以及m6A阅读器亲王室的重要性

• 批准号: 2878039
• 金额: --
• 依托单位: University of Leeds
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2023
• 资助国家: 英国
• 起止时间: 2023 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2878039
• 关键词: Elucidating; role; novel; virus; induced

The ability of non-coding RNAs (ncRNAs) to regulate gene expression offers unique possibilities for viruses to modulate virus gene expression. We have recently shown that the herpesvirus, KSHV, can manipulate the levels of host cell circular RNAs to enhance its own replication, by regulating the activity of other ncRNA species in so-called ncRNA regulatory networks. However, how KSHV regulates circRNA biogenesis to dysregulate their levels during infection is unknown. In this project I will examine (i) the role of virus-induced nuclear condensates in circRNA biogenesis and (ii) the role of host cell m6A machinery, particularly novel m6A reader proteins, utilised by viruses to regulate both viral and cellular ncRNA and mRNA processing during infection. Therefore, the project aims to better understanding the roles of ncRNA and m6A in virus replication and how gene expression is regulated post-transcriptionally during infection.

非编码rna （ncRNAs）调节基因表达的能力为病毒调节病毒基因表达提供了独特的可能性。我们最近表明，疱疹病毒KSHV可以通过调节所谓的ncRNA调节网络中其他ncRNA物种的活性来操纵宿主细胞环状rna的水平，以增强其自身的复制。然而，在感染期间，KSHV如何调节circRNA生物发生以失调其水平尚不清楚。在这个项目中，我将研究(I)病毒诱导的核凝聚物在环状rna生物发生中的作用，以及（ii）宿主细胞m6A机制的作用，特别是新型m6A读取器蛋白，病毒利用它在感染期间调节病毒和细胞ncRNA和mRNA的加工。因此，该项目旨在更好地了解ncRNA和m6A在病毒复制中的作用，以及感染过程中基因表达的转录后调控机制。