INFLUE HETEROCYCLIC AMINE METABOLIC POLYMORPH ON BIOACTIVAT PHIP IN HUMAN COLON

影响人结肠中生物活性剂 PHIP 的杂环胺代谢多晶型

• 批准号: 7183225
• 负责人: NICHOLAS PAUL LANG
• 金额: $ 1.24万
• 依托单位: UNIVERSITY OF CALIF-LAWRNC LVRMR NAT LAB
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-01 至 2006-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7183225
• 关键词: analytical method; biomedical equipment resource; clinical research; mass spectrometry; method development; technology /technique development

DESCRIPTION (provided by applicant): The National Resource for Accelerator Mass Spectrometry (AMS) was established in 1999 to enable biomedical researchers to accurately quantify very low levels of radioisotopes while exploring fundamental issues in biology. In this renewal, we will expand our present capabilities by developing methods to study biochemical pathways and cellular processes at the level of the single cell and small groups of cells, which will allow our collaborators to study the effect of aging in yeast. The Resource will also develop methods to quantify endogenous processes, such as protein and DNA oxidation in higher organisms. This will facilitate the study of inflammatory diseases ranging from atherosclerosis to neurodegenerative disorders to cancer. To further these goals, we will develop a sample presentation technology that will enable the analysis of much smaller samples than can be currently processed, as well as techniques for speciation of biomolecules in such samples. Throughout the tenure of the grant we will continue to provide a resource to the research community that will include service to investigators familiar with AMS, training of investigators in the technology and dissemination of the Resource. Towards these goals, our specific aims are to: 1.) Develop protocols for the online combustion of eluents from separatory instruments and couple the products to a gas accepting ion source to increase the sensitivity of AMS. 2.) Increase the value and information content of AMS measurements by quantitating isotope content in isolates of uniformly isotope-labeled systems or by quantitating derivatized biomarkers of modification or function on specific macromolecules. 3.) Provide quantitation of effector and effect in biological systems using multiple isotopic tracers within sampled materials. We will achieve this by developing a robust and higher throughput process for 3H sample preparation and by leveraging against other funded isotope measurements at the Center for Accelerator Mass Spectrometry (CAMS). 4.) Provide high throughput and precise quantitation for collaborative and service users.

描述（由申请人提供）：国家加速器质谱资源 (AMS) 成立于 1999 年，旨在帮助生物医学研究人员在探索生物学基本问题的同时准确量化极低水平的放射性同位素。在这次更新中，我们将通过开发在单细胞和小细胞群水平上研究生化途径和细胞过程的方法来扩展我们现有的能力，这将使我们的合作者能够研究酵母衰老的影响。该资源还将开发量化内源过程的方法，例如高等生物中的蛋白质和 DNA 氧化。这将促进从动脉粥样硬化到神经退行性疾病再到癌症等炎症性疾病的研究。为了进一步实现这些目标，我们将开发一种样品呈现技术，该技术将能够分析比目前可处理的样品小得多的样品，以及此类样品中生物分子形态的技术。在整个赠款期限内，我们将继续向研究界提供资源，其中包括为熟悉 AMS 的研究人员提供服务、对研究人员进行技术培训和资源传播。为了实现这些目标，我们的具体目标是： 1.) 开发分离仪器洗脱液在线燃烧的方案，并将产品与气体接受离子源耦合，以提高 AMS 的灵敏度。 2.) 通过定量统一同位素标记系统分离物中的同位素含量或定量特定大分子修饰或功能的衍生生物标志物，增加 AMS 测量的价值和信息内容。 3.) 使用多种同位素示踪剂对生物系统中的效应子和效应进行定量 在采样材料内。我们将通过开发强大且更高吞吐量的解决方案来实现这一目标 3H 样品制备过程，并利用加速器质谱中心 (CAMS) 的其他资助的同位素测量。 4.) 为协作和服务用户提供高通量和精确定量。