Core--Mammalian Cell Resource

哺乳动物细胞资源（MCR）核心在程序项目中有两个主要目标和几个功能。其两项主要职责是：(1)对在实施本项目研究计划过程中开发的哺乳动物细胞系以及项目负责人确定的需要更广泛表征的任何其他细胞系进行彻底和仔细的表征，以及(2)根据各个项目的要求，从特定细胞系中制备哺乳动物细胞提取物。此外，MCR核心将为所有项目提供保存细胞系作为冷冻保存样本的服务，并根据项目负责人确定的实验目的提供所需的细胞系。这些冷冻样品将来自具有表型特征并经证明不含支原体的细胞群。通过集中和保持对所有项目中使用的细胞系的质量控制，将促进整个项目中生化和遗传实验结果的比较，保证结果的可重复性，并使个别研究人员对项目间比较充满信心。MCR核心的目标是：(1)彻底和仔细地表征将在项目中广泛使用的所有细胞系的生长特性、克隆生存/细胞毒性和突变频率响应（例如，UVC、MMC、补骨脂素+ UVA、IR或其他必要的因素）、耐药性表型（例如，8-AA、6-TG、HAT、ALASA、G418等）和错配修复状态/错配修复表型（视情况而定）。然后将这些特征细胞系在培养中扩增，并从呈指数增长的种群中制备和编目大量冷冻保存的样本，以便按要求分发给各个项目；(2)根据个别项目的要求，从哺乳动物细胞系中制备小（2升）和大（最多50升）提取物，用于进行生化分析（例如，修复、切口、电泳迁移转移）。

The Mammalian Cell Resource (MCR) Core has two major goals and several functions in the Program Project. Its two major responsibilities are: (1) To thoroughly and carefully characterize the mammalian cell lines developed in the course of implementing the research plans within the Program Project, as well as any other cell lines that require more extensive characterization as determined by the Project Leaders, and (2) To prepare mammalian cell extracts from specific cell lines as required by the individual Projects. In addition, the MCR Core will provide the service to all Projects of maintaining cell lines as cryopreserved samples and supplying them on demand for the experimental purposes determined by the Project Leaders. These frozen samples will be derived from cell populations characterized phenotypically and certified free of mycoplasma. By centralizing and maintaining quality control of the cell lines to be used in all of the Projects, comparisons of the results of biochemical and genetic experiments across the Program Project will be facilitated, reproducibility of results will be assured, and individual investigators will be confident of inter-project comparisons. The aims of the MCR Core are: (1) To thoroughly and carefully characterize all cell lines that will be extensively used in the Program Project with respect to growth characteristics, clonogenic survival/cytotoxicity and mutation frequency responses (e.g., UVC, MMC, psoralen + UVA, IR, or others as needed), drug resistance phenotypes (e.g., 8-AA, 6-TG, HAT, ALASA, G418, etc.), and mismatch repair status/mismatch repair phenotypes, as appropriate. These characterized cell lines will then be expanded in culture and numerous cryopreserved samples from exponentially growing populations will be prepared and catalogued for distribution to the individual Projects as requested; and (2) To prepare both small (2-liter) and large (up to 50-liter) extracts from mammalian cell lines for the purposes of conducting biochemical assays (e.g., repair, incision, electrophoretic mobility shifts) as required by the individual Projects.