CORE--PREPARATIVE FACILITIES

核心——准备设施

• 批准号: 7464616
• 负责人: Zijian Xie
• 金额: $ 42.04万
• 依托单位: UNIVERSITY OF TOLEDO HEALTH SCI CAMPUS
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-03-01 至 2008-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7464616
• 关键词: Adult; Cardiac; Cardiac Myocytes; Cell membrane; Cells; Core Facility; Equipment; Family suidae; H(+)-K(+)-Exchanging ATPase; Heart Ventricle; Insecta; Kidney; Membrane; Na(+)-K(+)-Exchanging ATPase; Neonatal; Numbers; Preparation; Purpose; Rattus; Recombinants; Research; Scientist; Sus scrofa; Suspension substance; Suspensions; Work; beef; culture plates; research study

DESCRIPTION (provided by applicant) The purpose of this core remains the centralization of repetitive preparations of several items that are "starting materials" and are used by one or more of the projects. We have found this approach to be efficient and convenient for all concerned. It allows the core technicians to master a small number of difficult tasks; when one is temporarily absent or needs to be replaced the work does not stop; and there is a continuous supply of high quality material available to the users. The common core facility, equipment, and routine supplies, administered and supervised by a research scientist with appropriate expertise, is also quite economical. The preparations to be made by the core are: 1. Primary cultures of neonatal rat cardiac myocytes, and adult rat cardiac myocytes either in suspension or as plated cultures, to be used by Projects 1, 11, and some of the experiments of Project 12 that will be done in Toledo. 2. Preparations of crude Sf-21 insect cell membranes containing the recombinant H+/K+-ATPase to be used by Project 10. 3. Preparation of purified recombinant Src expressed in Sf-9 cells, to be used primarily by Project 10. 4. Preparations of cardiac sarcolemmal membranes from beef and rat heart ventricles, to be used primarily by Project 1. 5. Preparation of purified Na+/K+-ATPase from pig kidney, to be used primarily by Project 11, and to smaller extents by Projects 1 and 10.

描述（由申请人提供） 这个核心的目的仍然是集中几个项目的重复准备工作 它们是“起始材料”，被一个或多个项目使用。我们看这个 方法是有效和方便所有有关。它允许核心技术人员 掌握少量困难的任务;当某人暂时缺席或需要时 更换工作不停顿;并有优质材料源源不断的供应 提供给用户。共同的核心设施、设备和日常用品， 由具有适当专业知识的研究科学家管理和监督， 经济实惠。核心要做的准备是：1。新生大鼠原代培养物 心肌细胞和成年大鼠心肌细胞，悬浮培养或平板培养， 项目1、11和项目12的一些实验将在2012年完成， 位于托莱多2.含有重组体的粗Sf-21昆虫细胞膜的制备 H+/K+-ATP酶供项目10使用。 3.表达的纯化的重组Src的制备 SF-9细胞，主要用于项目10。 4.心肌肌膜制剂 牛和大鼠心室的膜，主要用于项目1。 5.制备 从猪肾中纯化的Na+/K+-ATP酶，主要用于项目11， 项目1和项目10的范围。