CORE--PREPARATIVE FACILITIES

描述(由申请人提供) 这个核心的目的仍然是集中几个项目的重复性准备 这是一个或多个项目所使用的“起始材料”。我们找到了这个 为所有有关各方提供高效和方便的方法。它允许核心技术人员 掌握少量困难任务；当一个人暂时缺席或需要离开时 更换工作不会停止；并且有高质量材料的持续供应 可供用户使用。共同的核心设施、设备和日常用品， 由一位具有适当专业知识的研究科学家管理和监督，也相当 很经济。中心将做的准备工作如下：1.新生大鼠原代培养 心肌细胞，以及悬浮培养或平板培养的成年大鼠心肌细胞， 将由项目1、11以及项目12中将在 托莱多。2.重组人SF-21昆虫细胞膜粗品的制备 项目使用的H/K-ATPase。3.纯化的重组Src的制备 SF-9细胞，主要用于项目10.4.心肌肌膜的制备 主要用于项目1.5的牛肉和大鼠心脏室壁膜的制备 从猪肾中提纯的Na/K-ATPase，主要由Project 11使用，并扩大到更小 按项目1和10列出的范围。

DESCRIPTION (provided by applicant) The purpose of this core remains the centralization of repetitive preparations of several items that are "starting materials" and are used by one or more of the projects. We have found this approach to be efficient and convenient for all concerned. It allows the core technicians to master a small number of difficult tasks; when one is temporarily absent or needs to be replaced the work does not stop; and there is a continuous supply of high quality material available to the users. The common core facility, equipment, and routine supplies, administered and supervised by a research scientist with appropriate expertise, is also quite economical. The preparations to be made by the core are: 1. Primary cultures of neonatal rat cardiac myocytes, and adult rat cardiac myocytes either in suspension or as plated cultures, to be used by Projects 1, 11, and some of the experiments of Project 12 that will be done in Toledo. 2. Preparations of crude Sf-21 insect cell membranes containing the recombinant H+/K+-ATPase to be used by Project 10. 3. Preparation of purified recombinant Src expressed in Sf-9 cells, to be used primarily by Project 10. 4. Preparations of cardiac sarcolemmal membranes from beef and rat heart ventricles, to be used primarily by Project 1. 5. Preparation of purified Na+/K+-ATPase from pig kidney, to be used primarily by Project 11, and to smaller extents by Projects 1 and 10.