Acquisition of a CD Spectrophotometer

购买 CD 分光光度计

• 批准号: 7046996
• 负责人: DALE F MIERKE
• 金额: $ 10.47万
• 依托单位: BROWN UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-06-01 至 2007-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7046996
• 关键词: buffers; computers; fluorescence; ligands; medicine; nucleic acids; peptides; performance; proteins; solvents; temperature; tryptophan; university

DESCRIPTION (provided by applicant): A consortium of investigators at Brown University in the Division of Biology & Medicine and the Departments of Physics, Chemistry, and Engineering is requesting support from the NCRR Shared Instrumentation Grant for purchase of a JASCO J-810 CD spectropolarimeter. At Brown CD is used to analyze a broad range of samples that include peptides, proteins, nucleic acids, and their complexes, providing unique information on structure, stability, and dynamics, including thermodynamic and kinetic parameters of these systems. The deteriorating performance (e.g., poor UV, below 200 nm, penetration) of the current CD instrument (approaching its 15th year in operation) places severe limitations on the scientific problems that can be undertaken. The requested instrument with computer controlled temperature variation, a syringe-based titration and stop-flow system, and simultaneous CD and fluorescence absorption will allow for a large number of experiments currently not possible. These include thermo-melting curves for stability determination, .accurate titrations of solvents, buffers, denaturants, ligands, or binding partners and the temporal determination of the structural variation induced by alteration of these variables. We will be able to correlate structural changes (CD) with binding events (e.g., as monitored by changes in tryptophan or use of fluorescent ligands) by the simultaneous acquisition of CD and fluorescence absorption. As detailed in this proposal, such studies are (or will become) an integral part of a number of NIH sponsored research projects and therefore the instrument will have an immediate and long-lasting impact on the biomedical research conducted at Brown University.

描述(由申请人提供)：布朗大学生物和医学系以及物理系、化学系和工程系的一个研究小组正在请求NCRR共享仪器基金的支持，以购买JASCO J-810 CD光谱偏振仪。在Brown CD用于分析各种样品，包括多肽、蛋白质、核酸和它们的络合物，提供有关结构、稳定性和动力学的独特信息，包括这些体系的热力学和动力学参数。当前CD仪器的性能恶化(例如，紫外线较差，低于200 nm，渗透率低于200 nm)(接近其运行的第15个年头)给可以解决的科学问题带来了严重的限制。所要求的仪器具有计算机控制的温度变化，基于注射器的滴定和停流系统，以及同时进行镉和荧光吸收，将允许进行目前不可能进行的大量实验。这些包括用于稳定性测定的热熔化曲线，溶剂、缓冲剂、变性剂、配体或结合伙伴的准确滴定，以及这些变量变化引起的结构变化的时间测定。我们将能够通过同时获取CD和荧光吸收来将结构变化(CD)与结合事件(例如，通过色氨酸的变化或荧光配体的使用来监控)相关联。如本提案所述，此类研究是(或将成为)美国国立卫生研究院赞助的许多研究项目的组成部分，因此该仪器将对布朗大学进行的生物医学研究产生直接和长期的影响。

项目成果

The Chirality of Aggregated Yariv Reagents Correlates with Their AGP-Binding Ability.

聚集的 Yariv 试剂的手性与其 AGP 结合能力相关。

• DOI: 10.1002/cbic.202100532
• 发表时间: 2022
• 期刊: Chembiochem : a European journal of chemical biology
• 作者: Hoshing,Raghuraj;Leeber3rd,BlaiseW;Kuhn,Helene;Caianiello,David;Dale,Brandon;Saladino,Michael;Lusi,Robert;Palaychuk,Natalie;Weingarten,Sarah;Basu,Amit
• 通讯作者: Basu,Amit

Thermodynamic consequences of the hyperoxidized guanine lesion guanidinohydantoin in duplex DNA.

• DOI: 10.1021/tx300190a
• 发表时间: 2012-08-20
• 期刊: Chemical research in toxicology
• 影响因子: 4.1
• 作者: Yennie CJ;Delaney S
• 通讯作者: Delaney S

A Functionalizable Analog of the Yariv Reagent for AGP Imaging using Fluorescence Microscopy

使用荧光显微镜进行 AGP 成像的 Yariv 试剂的功能化类似物

• DOI: 10.1021/acs.bioconjchem.3c00184
• 发表时间: 2023
• 期刊: Bioconjugate Chemistry
• 影响因子: 4.7
• 作者: Rueda, Sebastian;McCubbin, Tyler J.;Shieh, Meg;Hoshing, Raghuraj;Braun, David M.;Basu, Amit
• 通讯作者: Basu, Amit

Enzyme Efficiency but Not Thermostability Drives Cefotaxime Resistance Evolution in TEM-1 β-Lactamase.

• DOI: 10.1093/molbev/msx053
• 发表时间: 2017-05-01
• 期刊: Molecular biology and evolution
• 影响因子: 10.7
• 作者: Knies JL;Cai F;Weinreich DM
• 通讯作者: Weinreich DM

Heterologous high-level E. coli expression, purification and biophysical characterization of the spine-associated RapGAP (SPAR) PDZ domain.

• DOI: 10.1016/j.pep.2008.07.003
• 发表时间: 2008-11
• 期刊: Protein expression and purification
• 影响因子: 1.6
• 作者: Breann L Brown;M. Hadley;R. Page