Acquisition of a CD Spectrophotometer

购买 CD 分光光度计

基本信息

批准号：
7046996
负责人：
DALE F MIERKE
金额：
$ 10.47万
依托单位：
BROWN UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2006
资助国家：
美国
起止时间：
2006-06-01 至 2007-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7046996
关键词：
buffers computers fluorescence ligands medicine nucleic acids peptides performance proteins solvents temperature tryptophan university

项目摘要

DESCRIPTION (provided by applicant): A consortium of investigators at Brown University in the Division of Biology & Medicine and the Departments of Physics, Chemistry, and Engineering is requesting support from the NCRR Shared Instrumentation Grant for purchase of a JASCO J-810 CD spectropolarimeter. At Brown CD is used to analyze a broad range of samples that include peptides, proteins, nucleic acids, and their complexes, providing unique information on structure, stability, and dynamics, including thermodynamic and kinetic parameters of these systems. The deteriorating performance (e.g., poor UV, below 200 nm, penetration) of the current CD instrument (approaching its 15th year in operation) places severe limitations on the scientific problems that can be undertaken. The requested instrument with computer controlled temperature variation, a syringe-based titration and stop-flow system, and simultaneous CD and fluorescence absorption will allow for a large number of experiments currently not possible. These include thermo-melting curves for stability determination, .accurate titrations of solvents, buffers, denaturants, ligands, or binding partners and the temporal determination of the structural variation induced by alteration of these variables. We will be able to correlate structural changes (CD) with binding events (e.g., as monitored by changes in tryptophan or use of fluorescent ligands) by the simultaneous acquisition of CD and fluorescence absorption. As detailed in this proposal, such studies are (or will become) an integral part of a number of NIH sponsored research projects and therefore the instrument will have an immediate and long-lasting impact on the biomedical research conducted at Brown University.

描述（由申请人提供）：布朗大学生物学与医学系的调查人员财团以及物理，化学和工程部门要求提供NCRR共享仪器赠款的支持，以购买JASCO J-810 CD SpectroPolorimeter。在棕色CD上，用于分析包括肽，蛋白质，核酸及其复合物在内的广泛样品，提供了有关这些系统的热力学和动力学参数，提供了有关结构，稳定性和动力学的独特信息。当前CD仪器（即将在运营的第15年）的性能恶化（例如，较差的紫外线，低于200 nm，穿透）对可以进行的科学问题的严重限制。带有计算机控制温度变化的请求仪器，基于注射器的滴定和停止流系统，以及同时的CD和荧光吸收，将允许目前无法进行大量实验。这些包括用于确定稳定性的热熔曲线，准确地滴定溶剂，缓冲液，变性剂，配体或结合伴侣，以及通过改变这些变量引起的结构变化的时间确定。我们将能够通过同时获得CD和荧光吸收来将结构变化（CD）与结合事件（例如，通过色氨酸或使用荧光配体的使用而监测）。正如该提案中详细介绍的那样，此类研究是（或将成为）许多NIH赞助的研究项目的组成部分，因此该工具将对布朗大学进行的生物医学研究产生直接持久的影响。

项目成果

期刊论文数量（7）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

The Chirality of Aggregated Yariv Reagents Correlates with Their AGP-Binding Ability.

聚集的 Yariv 试剂的手性与其 AGP 结合能力相关。

DOI：
10.1002/cbic.202100532
发表时间：
2022
期刊：
Chembiochem : a European journal of chemical biology
影响因子：
0
作者：
Hoshing,Raghuraj;Leeber3rd,BlaiseW;Kuhn,Helene;Caianiello,David;Dale,Brandon;Saladino,Michael;Lusi,Robert;Palaychuk,Natalie;Weingarten,Sarah;Basu,Amit
通讯作者：
Basu,Amit

Thermodynamic consequences of the hyperoxidized guanine lesion guanidinohydantoin in duplex DNA.

DOI：
10.1021/tx300190a
发表时间：
2012-08-20
期刊：
Chemical research in toxicology
影响因子：
4.1
作者：
Yennie CJ;Delaney S
通讯作者：
Delaney S

A Functionalizable Analog of the Yariv Reagent for AGP Imaging using Fluorescence Microscopy

使用荧光显微镜进行 AGP 成像的 Yariv 试剂的功能化类似物

DOI：
10.1021/acs.bioconjchem.3c00184
发表时间：
2023
期刊：
Bioconjugate Chemistry
影响因子：
4.7
作者：
Rueda, Sebastian;McCubbin, Tyler J.;Shieh, Meg;Hoshing, Raghuraj;Braun, David M.;Basu, Amit
通讯作者：
Basu, Amit

Enzyme Efficiency but Not Thermostability Drives Cefotaxime Resistance Evolution in TEM-1 β-Lactamase.