Acquisition of a CD Spectrophotometer

购买 CD 分光光度计

• 批准号: 7046996
• 负责人: DALE F MIERKE
• 金额: $ 10.47万
• 依托单位: BROWN UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-06-01 至 2007-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7046996
• 关键词: buffers; computers; fluorescence; ligands; medicine; nucleic acids; peptides; performance; proteins; solvents; temperature; tryptophan; university

DESCRIPTION (provided by applicant): A consortium of investigators at Brown University in the Division of Biology & Medicine and the Departments of Physics, Chemistry, and Engineering is requesting support from the NCRR Shared Instrumentation Grant for purchase of a JASCO J-810 CD spectropolarimeter. At Brown CD is used to analyze a broad range of samples that include peptides, proteins, nucleic acids, and their complexes, providing unique information on structure, stability, and dynamics, including thermodynamic and kinetic parameters of these systems. The deteriorating performance (e.g., poor UV, below 200 nm, penetration) of the current CD instrument (approaching its 15th year in operation) places severe limitations on the scientific problems that can be undertaken. The requested instrument with computer controlled temperature variation, a syringe-based titration and stop-flow system, and simultaneous CD and fluorescence absorption will allow for a large number of experiments currently not possible. These include thermo-melting curves for stability determination, .accurate titrations of solvents, buffers, denaturants, ligands, or binding partners and the temporal determination of the structural variation induced by alteration of these variables. We will be able to correlate structural changes (CD) with binding events (e.g., as monitored by changes in tryptophan or use of fluorescent ligands) by the simultaneous acquisition of CD and fluorescence absorption. As detailed in this proposal, such studies are (or will become) an integral part of a number of NIH sponsored research projects and therefore the instrument will have an immediate and long-lasting impact on the biomedical research conducted at Brown University.

描述（由申请人提供）：布朗大学生物与医学系以及物理、化学和工程系的一个研究人员联盟正在请求 NCRR 共享仪器拨款支持购买 JASCO J-810 CD 分光旋光计。 Brown CD 用于分析各种样品，包括肽、蛋白质、核酸及其复合物，提供有关结构、稳定性和动力学的独特信息，包括这些系统的热力学和动力学参数。当前 CD 仪器（运行已接近 15 年）的性能不断恶化（例如，紫外线差、低于 200 nm、穿透力），严重限制了可以解决的科学问题。所要求的仪器具有计算机控制的温度变化、基于注射器的滴定和停流系统以及同步 CD 和荧光吸收，将允许进行目前不可能进行的大量实验。这些包括用于稳定性测定的热熔曲线、溶剂、缓冲剂、变性剂、配体或结合配偶体的准确滴定以及由这些变量的改变引起的结构变化的时间测定。通过同时采集 CD 和荧光吸收，我们将能够将结构变化 (CD) 与结合事件相关联（例如，通过色氨酸的变化或使用荧光配体进行监测）。正如该提案中所详述的，此类研究是（或将成为）美国国立卫生研究院资助的许多研究项目的组成部分，因此该仪器将对布朗大学进行的生物医学研究产生直接和长期的影响。

项目成果

The Chirality of Aggregated Yariv Reagents Correlates with Their AGP-Binding Ability.

聚集的 Yariv 试剂的手性与其 AGP 结合能力相关。

• DOI: 10.1002/cbic.202100532
• 发表时间: 2022
• 期刊: Chembiochem : a European journal of chemical biology
• 作者: Hoshing,Raghuraj;Leeber3rd,BlaiseW;Kuhn,Helene;Caianiello,David;Dale,Brandon;Saladino,Michael;Lusi,Robert;Palaychuk,Natalie;Weingarten,Sarah;Basu,Amit
• 通讯作者: Basu,Amit

Thermodynamic consequences of the hyperoxidized guanine lesion guanidinohydantoin in duplex DNA.

• DOI: 10.1021/tx300190a
• 发表时间: 2012-08-20
• 期刊: Chemical research in toxicology
• 影响因子: 4.1
• 作者: Yennie CJ;Delaney S
• 通讯作者: Delaney S

A Functionalizable Analog of the Yariv Reagent for AGP Imaging using Fluorescence Microscopy

使用荧光显微镜进行 AGP 成像的 Yariv 试剂的功能化类似物

• DOI: 10.1021/acs.bioconjchem.3c00184
• 发表时间: 2023
• 期刊: Bioconjugate Chemistry
• 影响因子: 4.7
• 作者: Rueda, Sebastian;McCubbin, Tyler J.;Shieh, Meg;Hoshing, Raghuraj;Braun, David M.;Basu, Amit
• 通讯作者: Basu, Amit

Enzyme Efficiency but Not Thermostability Drives Cefotaxime Resistance Evolution in TEM-1 β-Lactamase.

• DOI: 10.1093/molbev/msx053
• 发表时间: 2017-05-01
• 期刊: Molecular biology and evolution
• 影响因子: 10.7
• 作者: Knies JL;Cai F;Weinreich DM
• 通讯作者: Weinreich DM

Heterologous high-level E. coli expression, purification and biophysical characterization of the spine-associated RapGAP (SPAR) PDZ domain.

• DOI: 10.1016/j.pep.2008.07.003
• 发表时间: 2008-11
• 期刊: Protein expression and purification
• 影响因子: 1.6
• 作者: Breann L Brown;M. Hadley;R. Page