Advantages of HPLC with Ultra Stable Phases

具有超稳定相的 HPLC 的优点

• 批准号: 7047792
• 负责人: PETER W CARR
• 金额: $ 25.64万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-08-01 至 2008-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7047792
• 关键词: acidity /alkalinity; biomedical equipment development; catalyst; chemical structure; crosslink; high performance liquid chromatography; silicon compounds; surface coating; surface property; thermodynamics; thermostability

DESCRIPTION (provided by applicant): Previously, we showed how to make and use stable zirconia RPLC media, introducing two new techniques, thermally tuned tandem column (T3C) chromatography and high temperature ultra-fast liquid chromatography (HTUFLC). We now propose to make and characterize a number of innovative highly stable silica (SiO2) phases that will use hypercrosslinked surface polymer networks confined to a monolayer on the silica surface. We will then apply these materials to the high-speed analysis of pharmaceuticals, environmental and workplace toxicants, and ultimately, in collaboration with Professor David Muddiman, to complex biological samples. Our goal is to explore all aspects of the new derivatization technology, including conventional LC, packed capillary LC and capillary electrochromatography. We will explore two general concepts in HPLC made possible by the availability of high stability phases. First, we propose to develop fast, comprehensive two-dimensional HPLC (2D-LC) based on our phases. Due to the thermal limitations of conventional supports, the advantages of using high temperatures in HPLC have hardly been explored. In particular, upon increasing the temperature to 200 degrees C, the resulting 20-fold decrease in viscosity gives a nearly 20-fold increase in analysis speed. We will use the high speed of HTUFLC to increase the speed of 2D-LC and make it a much more practical routine method. The use of higher temperatures also facilitates the use of longer columns, especially in a capillary format, particularly for microdetection. Second, we propose to extend the isocratic T3C concept to gradient elution chromatography. Both approaches are predicated on the availability of phases of high thermal stability.

描述（由申请人提供）：以前，我们展示了如何制备和使用稳定的氧化锆RPLC介质，介绍了两种新技术，热调谐串联柱（T3 C）色谱法和高温超快速液相色谱法（HTUFLC）。我们现在提出了一些创新的高度稳定的二氧化硅（SiO2）相，将使用超交联的表面聚合物网络限制在二氧化硅表面上的单层，并进行表征。然后，我们将这些材料应用于药物，环境和工作场所毒物的高速分析，并最终与大卫穆迪曼教授合作，复杂的生物样品。我们的目标是探索新的衍生技术的各个方面，包括传统的LC，填充毛细管LC和毛细管电色谱。我们将探讨高效液相色谱中的两个一般概念，这两个概念是由高稳定性相的可用性而成为可能的。首先，我们建议开发快速，全面的二维HPLC（2D-LC）的基础上，我们的阶段。由于传统载体的热限制，在HPLC中使用高温的优点几乎没有被探索。特别是，当温度升高到200摄氏度时，粘度降低20倍，分析速度提高近20倍。我们将利用HTUFLC的高速度来提高2D-LC的速度，使其成为更实用的常规方法。使用较高的温度也有利于使用较长的柱，特别是毛细管形式，特别是用于微量检测。其次，我们建议将等度T3 C概念扩展到梯度洗脱色谱。这两种方法都是基于高热稳定性相的可用性。