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Barrier functions of the sugary cell coat: Understanding how extracellular signalling proteins and bacterial toxins navigate the cell surface

糖细胞外壳的屏障功能：了解细胞外信号蛋白和细菌毒素如何在细胞表面导航

基本信息

批准号：
2885385
负责人：
金额：
--
依托单位：
University of Leeds
依托单位国家：
英国
项目类别：
Studentship
财政年份：
2023
资助国家：
英国
起止时间：
2023 至无数据
项目状态：
未结题

来源：
https://gtr.ukri.org/projects?ref=studentship-2885385
关键词：
Barrier functions sugary cell coat

项目摘要

Virtually every cell in the human body is surrounded by a carbohydrate-rich coat called the glycocalyx. The glycocalyx has been implicated in a variety of fundamental molecular and cellular processes including its ability to guide cell communication and behaviour by modulating the access of the extracellular signalling proteins, such as chemokines, to the cell surface. It also plays an important protective role as a barrier against pathogens, yet some bacterial toxins and viruses exploit the glycocalyx to gain entry to the host cells. Despite the omnipresence of the glycocalyx in the human body and its functional importance, its role has been elusive owing to the incredible complexity and hence overlooked as the tools for its investigation were missing.The biochemical and biophysical mechanisms of selective binding and transport of proteins and pathogens within the glycocalyx remain poorly understood. In this project, we are aiming to develop a mechanistic understanding of molecular diffusion within the glycocalyx by developing experimental glycocalyx models and new methods to probe the selective transport across the glycocalyx in molecular detail. The results of this project will provide new tools to further our fundamental understanding of glycocalyx functions that may in the future help tackling infectious diseases and control immune responses.

实际上，人体内的每一个细胞都被一种叫做糖萼的富含碳水化合物的外衣所包围。糖萼参与了各种基本的分子和细胞过程，包括通过调节细胞外信号蛋白(如趋化因子)对细胞表面的访问来指导细胞沟通和行为的能力。它还发挥着重要的保护作用，作为对病原体的屏障，但一些细菌毒素和病毒利用糖萼进入宿主细胞。尽管糖萼在人体内无处不在，具有重要的功能，但由于其极其复杂的功能，其作用一直难以捉摸，因此由于缺乏研究其研究的工具而被忽视。蛋白质和病原体在糖萼内选择性结合和运输的生化和生物物理机制仍然知之甚少。在这个项目中，我们的目标是通过开发实验性的糖萼模型和新的方法来探索分子细节的选择性转运，从而从机制上理解糖萼内的分子扩散。该项目的结果将提供新的工具，进一步加深我们对糖基化反应功能的基本了解，这可能有助于未来应对传染病和控制免疫反应。