Understanding the role of non-coding RNAs in intercellular communication and how its potential for the detection and inhibition of pre-metastatic canc

了解非编码 RNA 在细胞间通讯中的作用及其检测和抑制转移前癌症的潜力

• 批准号: 2885885
• 金额: --
• 依托单位: University of Nottingham
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2023
• 资助国家: 英国
• 起止时间: 2023 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2885885
• 关键词: Understanding; role; non; coding; RNAs

The PhD builds upon the successful work of the group in identifying the mechanisms by which extracellular vesicles influence the metastasis of cancer to bone. Once thought to be little more than a way for cells to offload waste, extracellular vesicles (EVs) are now recognised to be a deliberate way for a cell to secrete cargos of RNA, DNA and proteins to reshape tissues and act as signal carriers. These somewhat overlooked organelles may hold the key tounderstanding how tissues and systems in our bodies communicate, particularly within complex diseases (e.g., cancer and dysfunctional immune cells).The project will explore the non-coding RNA elements of these EVs to determine the potential regulatory mechanisms they control. The project will be a mixture of data analysis to build experimental hypothesises of the types of signalling and regulatory mechanisms that are altered by these groups of non-coding RNAs, combined with molecular biology to validate these hypotheses within in vitro models of prostate cancer.The project will require the development of molecular biology techniques alongside the ability to analyse large datasets and create experimental hypothesise based on existing data sources. These skills provide an excellent multidisciplinary basis and fit within the BBSRCs remit of approaches.

博士学位建立在该小组在确定细胞外囊泡影响癌症转移到骨的机制方面的成功工作的基础上。细胞外囊泡（EV）曾经被认为是细胞卸载废物的一种方式，现在被认为是细胞分泌RNA，DNA和蛋白质的货物以重塑组织并充当信号载体的一种故意方式。这些有些被忽视的细胞器可能是理解我们体内组织和系统如何交流的关键，特别是在复杂疾病中（例如，该项目将探索这些EV的非编码RNA元件，以确定它们控制的潜在调控机制。该项目将是一个混合的数据分析，以建立实验假设的类型的信号和调节机制，改变了这些群体的非编码RNA，结合分子生物学，在前列腺癌的体外模型中验证这些假设。该项目将需要发展分子生物学技术，同时能够分析大型数据集，并根据现有的数据源这些技能提供了一个很好的多学科基础，并符合BBSRCs的方法范围。