CORE--PROTEIN
核心--蛋白质
基本信息
- 批准号:7312214
- 负责人:
- 金额:$ 5.79万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:
- 资助国家:美国
- 起止时间:至
- 项目状态:未结题
- 来源:
- 关键词:
项目摘要
Support is requested for Protein Production. While small-scale preparations of recombinant proteins using standard affinity purifications will be performed in the individual laboratories of the project, all projects require mg-amounts of highly purified recombinant and untagged proteins. The Core will provide technical and scientific support in two ways. First, it will optimize expression of proteins that either do not express at all or only at very low levels, including change of vectors, bacterial strains, co-expression of chaperones, or switching to an eukaryotic expression system. Second, the core will express and purify medium to large
scale amounts of proteins using an array of conventional and affinity-based chromatographic methods. The core is equipped with a bacterial fermenter for large-scale expression, two state-of-the-art automated FPLC-systems, and a miniaturaized FPLC (SMART). An array of physico-chemical techniques will be used for initial characterization with respect to folding, oligomerization/aggregation and purity including CD-spectroscopy, multi-angle laser light scattering, and mass spectrometry. It is directd jointly by Drs. Fasshauer and Jahn, who will be assisted by M. Boeddener, an experienced Research Associate with several years of
experience in all of the required techniques.
要求支持蛋白质生产。虽然使用标准亲和纯化的重组蛋白的小规模制备将在项目的各个实验室进行,但所有项目都需要大量的高度纯化的重组蛋白和未标记的蛋白。核心将通过两种方式提供技术和科学支持。首先,它将优化完全不表达或仅表达非常低水平的蛋白质的表达,包括改变载体,菌株,伴侣蛋白的共表达,或切换到真核表达系统。第二,核心将表达和净化中至大
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Reinhard Jahn其他文献
Reinhard Jahn的其他文献
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{{ truncateString('Reinhard Jahn', 18)}}的其他基金
Assembly, structure, and function of activated SNARE complexes and their regulation by NSF/αSNAP, Munc18, Munc13, complexin, and synaptotagmin
激活的 SNARE 复合物的组装、结构和功能及其 NSF/αSNAP、Munc18、Munc13、complexin 和 synaptotagmin 的调节
- 批准号:
10202628 - 财政年份:2005
- 资助金额:
$ 5.79万 - 项目类别:
RECONSTITUTION OF SNARE-MEDIATED FUSION WITH NATIVE AND ARTIFICIAL MEMBRANES
圈套介导的天然膜和人工膜融合的重建
- 批准号:
7036463 - 财政年份:2004
- 资助金额:
$ 5.79万 - 项目类别:
RECONSTITUTION OF SNARE-MEDIATED FUSION WITH NATIVE AND ARTIFICIAL MEMBRANES
圈套介导的天然膜和人工膜融合的重建
- 批准号:
7797456 - 财政年份:
- 资助金额:
$ 5.79万 - 项目类别:
Assembly, structure, and function of activated SNARE complexes and their regulation by NSF/αSNAP, Munc18, Munc13, complexin, and synaptotagmin
激活的 SNARE 复合物的组装、结构和功能及其 NSF/αSNAP、Munc18、Munc13、complexin 和 synaptotagmin 的调节
- 批准号:
9209669 - 财政年份:
- 资助金额:
$ 5.79万 - 项目类别:
RECONSTITUTION OF SNARE-MEDIATED FUSION WITH NATIVE AND ARTIFICIAL MEMBRANES
圈套介导的天然膜和人工膜融合的重建
- 批准号:
7312209 - 财政年份:
- 资助金额:
$ 5.79万 - 项目类别:
Project 1- Reconstitution of SNAR-mediated Fusion with Native and Artificial Mem
项目 1 - 重建 SNAR 介导的与天然和人工 Mem 的融合
- 批准号:
8376187 - 财政年份:
- 资助金额:
$ 5.79万 - 项目类别:
RECONSTITUTION OF SNARE-MEDIATED FUSION WITH NATIVE AND ARTIFICIAL MEMBRANES
圈套介导的天然膜和人工膜融合的重建
- 批准号:
7596910 - 财政年份:
- 资助金额:
$ 5.79万 - 项目类别: