Cytokines and angiotensin II-induced hypertension

描述(由申请人提供)： 高血压是一个复杂的疾病过程，涉及许多病理生理变化。在这个计划项目中，个人研究主题集中在细胞因子在血管紧张素II诱导的高血压中参与异常血压调节的主题。该小组的策略是监测高血压发展过程中的许多相关变量，目的是确定从引入干预措施(血管紧张素II或笼子开关应激)到由此导致的血压升高的完整事件序列。这些变量包括神经和内分泌因素、细胞事件以及分子和基因特征的变化。来自两个行政单位的五名首席调查员已加入研究这些变化。动物模型将包括小鼠和大鼠(实验性高血压和转基因)。测量的具体变量包括：特定蛋白质的mRNA、酶活性、遗传关联、血管反应性、血压、激素水平、内皮衍生产品、细胞因子、钙、活性氧种和花生四烯酸代谢产物。研究战略将利用其中两个核心单元(核心A，动物和核心B，生物化学)中描述的专业知识和设备。生物统计支助和科学管理将通过行政股进行协调。从这些综合项目中，将更好地了解导致高血压的因素。

DESCRIPTION (provided by applicant): Hypertension is a complex disease process involving many pathophysiological changes. In this program project, the individual research topics focus on the theme that cytokines contribute to abnormal blood pressure regulation in angiotensin II-induced hypertension. The strategy of the group is to monitor many relevant variables during the development of hypertension with the goal of defining the complete sequence of events from the introduction of an intervention (angiotensin II or cage switch stress) to the resultant elevation in blood pressure. Among these variables are alterations in neural and endocrine factors, cellular events and molecular and gene characteristics. Five principal investigators from two administrative units have joined to study these changes. Animal models will include mice and rats (experimental hypertension and genetically modified). Among the specific variables measured will be the following: mRNA for specific proteins, enzyme activities, genetic associations, vascular reactivity, blood pressure, hormone levels, endothelium-derived products, cytokines, calcium, reactive oxygen species and arachidonic acid metabolites. Research strategies will utilize the professional expertise and equipment described in two of the core units (Core A, Animal and Core B, Biochemistry). Biostatistical support and scientific management will be coordinated through the Administrative Unit. From these integrated projects, a better understanding of the factors contributing to hypertension will emerge.