Identification of M. tuberculosis Lipid Kinases

描述（由申请人提供）：结核病感染了大约三分之一的世界人口，是一个全球性的健康问题。病原性分枝杆菌阻止吞噬体成熟并在宿主巨噬细胞内建立细胞内小生境的能力是感染的标志。发生这种情况的机制还不清楚。假设M.结核病主动分泌参与吞噬体成熟停滞的效应物。这些因子可以是蛋白质或脂质激酶，其将被递送到吞噬体腔，在那里它们可以靶向并调节正常的宿主信号传导和细胞内运输。本项目的目标是确定M。结核病效应激酶。该提案的具体目标是：1.对M.结核病与M.结核空泡。2.评估重组效应蛋白对宿主细胞的活性。

DESCRIPTION (provided by applicant): Tuberculosis infects roughly one third of the world population and is a global health concern. The ability of pathogenic mycobacteria to arrest phagosome maturation and establish an intracellular niche within the host macrophage is a hallmark of the infection. The mechanism by which this occurs is not well understood. It is hypothesized that M. tuberculosis actively secretes effectors involved in the arrest of phagosome maturation. Such factors could be protein or lipid kinases that would be delivered to the phagosome lumen where they could target and modulate normal host signaling and intracellular trafficking. The goal of this project is to identify M. tuberculosis effector kinases. The specific aims of this proposal are: 1. The identification of ATP-hydrolyzing enzymes secreted by M. tuberculosis and associated with the M. tuberculosis vacuole. 2. Assessing the activity of recombinant effector proteins on the host cell.