Rotation 1: Micropatterns of mouse and human motor neurons

旋转 1：小鼠和人类运动神经元的微模式

• 批准号: 2888227
• 金额: --
• 依托单位: University of Cambridge
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2023
• 资助国家: 英国
• 起止时间: 2023 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2888227
• 关键词: Rotation; Micropatterns; mouse; human; motor

BBSRC strategic theme: Understanding the rules of lifeAs an embryo begins to develop, early cell fate decisions produce extra-embryonic cells critical for the survival and development of the embryo proper. One of these lineages is the hypoblast, which controls the formation of the yolk sac, axial differentiation, and gastrulation. Despite the conservation of this cell type across amniotes and fish, little is known about how this cell type is initiated and maintained, and how this may have been conserved or have evolved between extant lineages. Therefore, this research project will investigate the conservation and function of transcription factors controlling this important cell lineage. I will utilise a range of stem cell models and embryos from across the animal kingdom, including humans, non-human primates, rodents, ungulates, amphibians, and fish. I will integrate genetic perturbation, advanced imaging, and multi-omics methods to investigate the conservation of molecular programmes in the hypoblast. Overall, this project will broaden our understanding of conserved molecular pathways that govern some of the very first cell fate decisions that shape embryogenesis.

BBSRC战略主题：了解生命的规则当胚胎开始发育时，早期细胞命运的决定产生了对胚胎本身的生存和发育至关重要的胚外细胞。这些谱系之一是下胚层，它控制着卵黄囊的形成、轴向分化和原肠胚形成。尽管这种细胞类型在蝾螈和鱼类中是保守的，但人们对这种细胞类型是如何启动和维持的，以及这种细胞类型是如何在现存谱系之间保守或进化的知之甚少。因此，本研究项目将研究控制这一重要细胞谱系的转录因子的保守性和功能。我将利用来自动物王国的一系列干细胞模型和胚胎，包括人类，非人类灵长类动物，啮齿动物，有蹄类动物，两栖动物和鱼类。我将整合遗传扰动，先进的成像技术，和多组学方法来研究下胚层的分子程序的保护。总的来说，这个项目将扩大我们对保守分子途径的理解，这些分子途径控制着一些塑造胚胎发生的最初细胞命运决定。